Observation 211258: Amanita sect. Vaginatae sensu Zhu L. Yang

When: 2015-07-23

Collection location: Allegany State Park, Cold Spring, Cattaraugus Co., New York, USA [Click for map]

41.9985° -78.9364° 450m

Who: Garrett Taylor (cappy)

Specimen available

Mixed woods, mostly deciduous. Tall as scissors. Cap broke. It had a washed out looking brown zone and a dark center. Thinking it’s the same as some I have been finding, but I don’t know if this one could be A. magna as it has this ring I described around the cap just behind the striate margin.

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nrLSU fragments
By: R. E. Tulloss (ret)
2018-01-12 15:29:47 PST (-0800)

We obtained several fragments of the nrLSU (nuclear ribosomal Large Subunit) gene. When matched to our local data base of nrLSU sequences from the Vaginatae both posted in GenBank or not posted, one fragment of 410 characters from an area of the gene reported as more highly conserved indicate high similarity to A. albemarlensis (#1), A. semiobruta (#2), and A. penetratrix (#3). A smaller fragment of about 170 characters from a different part of the nrLSU gene showed similarity of 100% (within a region 100% overlapping our fragment) to (only) sequences of the same three species listed previously.

Hence, we again have very good evidence that the voucher of the present observation is a species falling in one of the clusters that is part of the provisional Penetratrices.

I hope we do better on a second try or when trying the sequencing of a different gene.

At least on a first try we only got fragments of the “proposed fungal barcode”…
By: R. E. Tulloss (ret)
2018-01-12 14:54:19 PST (-0800)

gene sequence. However, one of the fragments shows that this taxon would be in provisional “Amanita series Penetratrices.” It would be very valuable to have new material of this taxon. More as we work through the recently received sequence data.

Very best,


Thank you, Garrett.
By: R. E. Tulloss (ret)
2015-07-25 04:16:02 PDT (-0700)

Very best,


I will bring it to NEMF.
By: Garrett Taylor (cappy)
2015-07-24 20:43:25 PDT (-0700)
Hello, Garrett.
By: R. E. Tulloss (ret)
2015-07-24 06:09:00 PDT (-0700)

I would be very interested in investigating this material. Thanks for posting it.

Very best,


Created: 2015-07-23 18:53:33 PDT (-0700)
Last modified: 2018-01-12 17:55:36 PST (-0800)
Viewed: 69 times, last viewed: 2018-01-13 02:06:27 PST (-0800)
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