Observation 213759: Lactarius Pers.

Proposed Names

77% (2)
Eye3 Eyes3
Recognized by sight: Looks like a pale version of L. petersenii

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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Okay, thanks
By: I. G. Safonov (IGSafonov)
2017-01-19 17:57:54 COT (-0500)

So two of the hits are actual vouchers and both are European species. They look somewhat similar to your mushroom, so at least there is some connection between the morphology and the sequences that makes sense.
I am a bit surprised nothing lit up from this continent, but there could be a number of reasons for it. Looks like it’s not petersenii and that one is in GenBank.
Don’t know anything about the phylogeny of milk caps, so to me it looks like a dead end without any further inputs. But, at least, the sequence has a “face”, which is not the case with the vast majority of GB uploads.

Top 5
By: Stephen Russell (Mycota)
2017-01-19 00:56:19 COT (-0500)

Select seq gb|EU819487.1| Lactarius sp. tjv1 voucher JMP0047 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence 1188 1188 100% 0.0 97% EU819487.1

Select seq gb|KF432964.1| Lactarius romagnesii voucher BG2011-32 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence 1146 1146 100% 0.0 96% KF432964.1

Select seq gb|JF519140.1| Uncultured Lactarius clone RELIS_K2_E07 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence 1138 1138 99% 0.0 96% JF519140.1

Select seq emb|HE820611.1| Uncultured ectomycorrhizal fungus genomic DNA containing ITS1, 5.8S rRNA gene, ITS2, clone morpho_623 1127 1127 94% 0.0 97% HE820611.1

Select seq gb|KF432962.1| Lactarius acris voucher BG2011-31 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence 1120 1120 100% 0.0 96% KF432962.1

Thanks for the info
By: I. G. Safonov (IGSafonov)
2017-01-19 00:21:08 COT (-0500)

Was it nrITS? What are the top 3-5 hits when the list is sorted by identity?

Not close to any named Lactarius in GenBank.
By: Stephen Russell (Mycota)
2017-01-18 23:38:14 COT (-0500)

Created: 2015-08-23 20:11:50 COT (-0500)
Last modified: 2017-01-18 23:37:42 COT (-0500)
Viewed: 54 times, last viewed: 2017-02-19 12:05:13 COT (-0500)
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