Observation 215566: Agrocybe Fayod
When: 2015-07-10
(42.6412° -80.5696° 203m)

Notes:
private tall grass prairie restoration
growing singly, found a few times
thin stem
cap about 1cm across
brown spores and gills

spores ovoid-oval-elliptical, with germ pore, smooth
gill trama parallel
cap cuticle filamentous (? I’m not confident in my ability to tell, section/setup micro properly)

Psilocybe, Stropharia, and Tubaria have filamentous cuticle
Agrocybe, Psathyrella, and Panaeolus would’ve been my guess, but cuticle should be cellular then
Need to look into this more, and other features

I mistook earlier for another Psathyrella ammophila (also present in this prairie)
prelim sequencing matches to Agrocybe

Species Lists

Images

559560
dissecting microscope on dried specimen
554358
in situ
559561
dissection gills closeup
559562
dried cap surface
559568
parallel gill trama (gill c.s., phase contrast)
559569
brown ovoid-oval-elliptical spores
559570
phase contrast spores, you can see there’s that pore opening part where they broke off their sterigmata… germ pores! no ornamentations
559571
cap cuticle seems filamentous (from an “oblique”? section of cap)
559572
filamentous cap cuticle (ignore the blurry under-layer and you can see the texture clearly)

Proposed Names

-45% (2)
Recognized by sight
28% (1)
Recognized by sight
-28% (1)
Recognized by sight: general appearance and habitat (sandy littery grassland)
Based on microscopic features: filamentous cuticle, spores brown oval smooth
2% (2)
Based on microscopic features: Spores with a germ pore. Pileipellis type excludes Conocybe and Agrocybe.
-28% (1)
Used references: better specimens later found on site that were sequenced…
56% (1)
Eye3 Eyes3
Used references: Partial sequence for 28S(LSU) of 611bp matches strongly with Agrocybe spp… 99% coverage and 99% identity for Agrocybe praecox, pediades, and smithii
e.g. Accession KC176295 A. smithii T-792 from Thorn et al 2012
https://www.ncbi.nlm.nih.gov/...

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Eye3 = Observer’s choice
Eyes3 = Current consensus

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Created: 2015-09-07 08:59:32 MST (-0700)
Last modified: 2016-06-15 12:46:36 MST (-0700)
Viewed: 76 times, last viewed: 2016-11-13 19:36:43 MST (-0700)
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