Notes: A single fresh specimen that popped up a day or two ago despite dry conditions. It was growing on the campus under an old oak. Apparently the shade provided by the luscious canopy and moisture from the morning dew was sufficient to promote fungal growth. I collected it just before I left the COMA foray, so this specimen didn’t make it to the ID tables.
> Stipe/pore surface stained blue instantly and erratically. The staining eventually resolved to pale grayish-brown
> The context was yellow, instantly staining blue; the bluing on cut context eventually resolved into reddish-brown discoloration (see last 2 pix)
> The odor was fungoid, not like curry or beef bouillon.
DNA Sequencing Discussion:
> A clean and contiguous 1370 bps nrLSU sequence was obtained for this material; the first 75 bps are missing though (the typical LSU sequences I get are 1440-1450 bps long).
> Not surprisingly, plugging the full-length sequence into GenBank’s BLAST didn’t return any meaningful hits. I’ve seen this happen to the few boletes for which DNA discussions were already posted on MO.
> A partial nrLSU sequence search (the first 974 characters) returned several results that make much more sense. The top hit (score = 1596; 867/870 overlay, 89% coverage; 99+% identity match) was actually L. pseudosensibilis voucher DS615-07 [GenBank accession #KF030257]. The second and third hits were B. pallidoroseus and B. bicolor vouchers, respectively [accession #HQ161860 and #KF030258].
|I’d Call It That||3.0||11.03||2||(Dave W,IGSafonov)|
sum(score * weight) /
(total weight + 1)
After running more “tests”, I upgraded my ID confidence. I think my species concept of fresh & young pseudosensibilis is now pretty much complete. Note the sky-blue (moderate blue) surface staining, the thin, bicolor-like tubes & their smooth transition into the stipe (just like in bicolor), and the context bluing resolving into a reddish-brown oxidation (this feature was pointed out by Gary Lincoff at the foray). As expected for pseudosensibilis, ammonia on cap gave a prominent violet-blue halo around the droplet and orange-brown discoloration under it.
pseudosensibilis is a slow stainer, with cap color often tending toward red sans brown overtones. But IMO the example seen here looks like classic pseudosensibils.
Created: 2015-09-07 15:22:43 PDT (-0700)
Last modified: 2016-01-23 18:13:53 PST (-0800)
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