Observation 215762: Porphyrellus porphyrosporus (Fr.) E.-J. Gilbert
When: 2015-09-06
Herbarium specimen reported

Notes: > Collected (not by me) at the COMA foray (site #8)
> Small and dark fruiting bodies; caps ~1.25" in diameter
> Unknown habitat, but presumably of mixed coniferous woods
> Cap flesh slowly staining blue
> This collection has been preserved
> The ID sticker says nebulosus, but this is likely to be incorrect

DNA Discussion:
> A clean and contiguous LSU sequence of 1461 bp was procured for this material.
> A 99% identity match (97% coverage, 1413/1419 bp contiguous sequence with 1 gap) with Porphyrellus porphyrosporus voucher collected in Germany (strain MB 97-023, GenBank accession DQ534643) was found via a GenBank BLAST search. The associated reference =
Binder, M. and Hibbett, D.S.; “Molecular systematics and biological diversification of Boletales” Mycologia 2006, 98 (6), 971-981.
> Interestingly, there were no GenBank hits (for LSU locus) associated with any North American collections of Porphyrellus.

Proposed Names

28% (1)
Recognized by sight
85% (1)
Eye3 Eyes3
Based on chemical features: Matching LSU sequence in GenBank — DNA discussion is posted in the notes section

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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Thank you, Dave and John
By: I. G. Safonov (IGSafonov)
2016-01-04 20:48:16 PST (-0800)

It’s hard to believe that no LSU sequences from P. porphyrosporus from the USA were recovered from the GenBank nucleotide search. Perhaps none exist as all NA collections have been sequenced only for ITS instead. The matching sequence from the German collection was used by both Nuhn et al. and Wu et al. in their recent phylogenetic papers on the Boletaceae from 2013 and 2014, respectively. This fact adds credence to this result and ID. In addition, Nuhn lists another LSU sequence (KF030346) that came from a second German collection of P. porphyrosporus, but for some unknown reason this one didn’t get picked up by the BLAST search. Roy Halling suggested I submit the LSU sequence from my material to GenBank as there is no coverage for NA specimens. I heard the submission process hadn’t been a trivial task, but maybe things got simpler over time. This is something to think about…

very nice
By: John Plischke (John Plischke)
2016-01-04 19:47:14 PST (-0800)

Very nice collection and notes

Nice job, Igor.
By: Dave W (Dave W)
2016-01-04 16:50:54 PST (-0800)

I often wonder about the possibility that eastern NA edulis material represents an array of native NA and imported Euro species. So, maybe this hypothesis also applies to other groups (clades?) of boletes?

DNA discussion posted
By: I. G. Safonov (IGSafonov)
2016-01-03 22:20:28 PST (-0800)

Created: 2015-09-08 21:02:02 PDT (-0700)
Last modified: 2016-01-03 22:19:44 PST (-0800)
Viewed: 75 times, last viewed: 2016-12-05 00:46:44 PST (-0800)
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