Observation 215899: Boletaceae Chevall.

When: 2015-09-11

Collection location: Tsukuba, Ibaragi, Japan [Click for map]

Who: lxjfx

Specimen available

Notes:
Growing under hardwoods

Species Lists

Images

555402
IMG_20150911_124725.jpg
555401
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555403
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555404
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555405
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555711
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Proposed Names

13% (3)
Eye3
Recognized by sight
19% (2)
Recognized by sight: B. subvelutipes is a name from the USA and is unlikely to occur in Japan.
28% (1)
Eyes3
Recognized by sight: Probably in Neoboletus-Sutorius “group”
Based on chemical features: nrITS sequence & BLASTn results

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

Add Comment
DNA sequencing results and discussion
By: I. G. Safonov (IGSafonov)
2018-01-22 19:53:41 PST (-0800)

> Originally, TEF-1-alpha locus was requested. However, Dr. Kudzma reported that sequence reads were “a useless mess”. Attempts to obtain a nrLSU sequence also met with failure due to exceptional heterogeneity that precluded getting contiguous reads.
> The derived nrITS sequence is 757 bps long. But even here things are not smooth either! The contiguous read starts in the middle of the ITS1F primer (in the 5’—>3’ direction) and continues without much of a hitch through all of ITS1 and 5.8S regions and into the first 103 bases of ITS2 (for a total of 567 characters, of which only 3 are ambiguous) before encountering a strongly heterogeneous/ambiguous area of 122 bps. The sequence then suddenly becomes specific again to provide the last readable 68 bases of ITS2 and the beginning of nrLSU. It’s interesting that all of the ITS haplotypes in this organism are of exactly the same length (!), which allows for a contiguous read in both directions.
> A BLASTn search of the ITS sequence (the first 567 bps) yielded no matches or close hits. The highest achieved similarity is only ~94% with Neoboletus sp. ‘flammeus’, a red-pore bolete endemic to western USA. The hit list mostly consists of accessions of taxa of Neoboletus/Sutorius, Rubroboletus, Suillellus, and Caloboletus.

Created: 2015-09-11 07:00:12 PDT (-0700)
Last modified: 2018-01-22 19:53:41 PST (-0800)
Viewed: 102 times, last viewed: 2018-04-05 22:23:07 PDT (-0700)
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