Observation 217124: Boletus variipes var. fagicola A.H. Sm. & Thiers
When: 2015-09-25
(43.2382° -77.5661° )

Notes: A single immature fruiting body growing in grass under an old oak and other hardwoods.

Molecular Sequencing Results:
> A clean, contiguous 1448 bp nrLSU sequence was obtained for this material
> Curiously, unlike my previous experience, a GenBank BLAST search of the full-length sequence gave a top scoring hit that made some sense — Boletus hiratsukae. This bolete is a bona fide member of the porcini sensu stricto and a relative of B. variipes from Japan. Its sequence of 1244 bp was long enough to show up in the search.
> A GenBank BLAST search of a 923 bps sequence fragment returned two very close matches with B. variipes var. fagicola holotype voucher collected/identified by A. H. Smith in 1968 in MI, accession #JQ327014 (score = 1613; 878/880 bp overlay, 99.8% similarity; 1 gap) and with B. variipes voucher collected/identified by T. M. Dentinger, accession #HQ161846 (score = 1602; 872/875 bp overlay, 99.7% similarity, no gaps). Interestingly, the gap in Smith’s voucher sequences was due to a missing cytocine at the end of the sequence fragment; that nucleotide was present in Dentinger’s voucher sequence.
Even without the DNA evidence, placement of this bolete into the variipes group made a lot of sense. The molecular evidence based on a single locus sequence gives a slight preference to var. fagicola.

Proposed Names

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Eye3 = Observer’s choice
Eyes3 = Current consensus


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Nice to see
By: walt sturgeon (Mycowalt)
2016-02-03 23:18:32 CST (-0600)

DNA almost confirms a macro identification, I think!

very good work
By: John Plischke (John Plischke)
2016-02-03 23:13:56 CST (-0600)

very good information to know. Glad to hear you are getting good results.

DNA discussion posted
By: I. G. Safonov (IGSafonov)
2016-02-03 22:13:25 CST (-0600)
By: I. G. Safonov (IGSafonov)
2016-01-02 01:09:51 CST (-0600)

Your proposal did cross my mind when I was posting this observation; however, I didn’t think this specimen was sufficiently dark enough to pass as var. fagicola.
I submitted this one for sequencing (the LSU locus) a month ago. I don’t know if one non-protein coding gene is enough to separate variipes from variipes var. fagicola at the species level. I suspect it’s not, but I should get more info from Roy Halling to be certain.
In the humongous bolete LSU tree published in the Nuhn et al. 2013 paper, the two species form a distinct and well-supported clade within the Boletus sensu stricto group, meaning that they are “siblings”. The same paper lists the holotype voucher of var. fagicola collected by A. H. Smith in 1968 for which LSU, Tef1 and RPB1 sequences exist.

Created: 2015-09-28 01:32:19 CDT (-0500)
Last modified: 2016-02-03 22:12:58 CST (-0600)
Viewed: 73 times, last viewed: 2016-10-22 02:37:39 CDT (-0500)
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