Observation 21760: Pluteus Fr.
When: 2009-06-05
No herbarium specimen

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By: Irene Andersson (irenea)
2009-06-09 08:48:47 CEST (+0200)

The first thing to look for, is pleurocystidia with or without thick walls and a hooked apex. I beleive you would have noticed if there were any. In the second microshot it looks like there could be thin-walled, fusoid cystida.

Next to look at, is the cap structure, either a cutis (thin, parallel hyphae, lying down), some with projecting ends (trichoderm) or hymeniform (a cover of globose cells, in some species mixed with more fusoid ones). From the looks of the cap, I guess this one has a cutis with projecting, elongated cells of some kind.

Have a look at this, and see if you can compare with it:

Some more pictures and descriptions here:

By: Shane Marsh (Mushane)
2009-06-09 06:39:06 CEST (+0200)

ill just give a good description for now. crumples up drawings

the spores showed characteristics you could cal obovoid, broadly ellipsoid, and even phaseoliform, in that some were rather large and round and others almost like a jelly bean.
the spores had 1-3 guttules, mostly just 1, and had thickened walls.

I couldnt find any clamp connections after many crush mounts of cap tissue and 45mins of hunting, finding clamps is always a task though right?

By: Shane Marsh (Mushane)
2009-06-08 21:55:31 CEST (+0200)

thanks christian, ill give it a shot.
mind you im not even good at drawing a straight/smooth line, ill see what I can produce.

again, no real structures, just spores, so the drawings will be limited.
ive also no graticule or micrometer.

Your welcome to a sample of this of you want some cap/gill pieces, I live downtown and your probably within a bike-ride away.

By: Christian (Christian Schwarz)
2009-06-08 21:17:19 CEST (+0200)

Hey Shane,
When you are at the mercy of your equipment, it is better to make drawings than post microphotos. Even with the best equipment, the microscope’s field of focus is so narrow that the shapes of structures are always more clear in the mind’s eye of the direct observer.
Learn to make good drawings (while observing, not from memory). They don’t have to be artistic, just clear. THe hardest part about learning to make micro-drawings is deciding how to make sense of the structure that you are seeing and distill it into a clear picture for the rest of us.
It helps most to look at the drawings of professionals, wherever you can find them.

Good luck!

By: Shane Marsh (Mushane)
2009-06-08 21:06:24 CEST (+0200)

this definitely does not match up to anything ive looked at so far.

anyone have any ideas, or is there something more I could provide as far as microscopy?
sorry about the shitty pics, I should have got the trinocular scope, but for now thats the best I can do.

By: Irene Andersson (irenea)
2009-06-06 23:02:55 CEST (+0200)

Not any of those three.
Looking forward to micro photos :-)

By: Shane Marsh (Mushane)
2009-06-06 22:42:29 CEST (+0200)

I could not locate any cystidia! (and it didnt bruise any specific color)

I found one thing sticking out on the edge of a gill but its HUGE and looks more like a piece of injured tissue.
I tried various mountants and still no cystidia, even a crush mount.
ALL cervinus that ive checked out are CHOCK LOADED with horned cystidia on all edges of the gill.

Both cervinus and salicinus have horned cystidia, im lost here.
That even leaves out petasatus!
Am I just missing something here or this not any of those 3?

I’ll post microscopy photos of this soon.

By: Shane Marsh (Mushane)
2009-06-06 17:13:12 CEST (+0200)

I locked this specimen in my car, with my keys hanging from the ignition LOL
im awaiting to see if it bruised any, it resembled salicinus, but its probably just cervinus.

I wish there was an easier Microscopic way to differentiate between salicinus and cervinus, but unfortunately I dont have a micrometer or a graticule so measuring spore/cystidia is out of the question.

Created: 2009-06-06 16:16:43 CEST (+0200)
Last modified: 2009-06-06 16:16:43 CEST (+0200)
Viewed: 91 times, last viewed: 2016-10-22 01:21:26 CEST (+0200)
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