Collection location: Los Trancos Preserve, Palo Alto, California, USA [Click for map]
Found on the bark of live oak.
The micro-shot is of the gill edge at 400x in KOH. The metuloid cystidia here are numerous. The cystidia are kinda interestingly encrusted, many encrusted in the top half, ending in a hard line.
Adding more micro details:
The second micro-shot is of the squashed gill edge at 1000x in congo red. Here are the smaller cystidia with a single projection.
The third micro-shot is of the squashed gill face at 1000x in congo red. Here are the encrusted metuloid cystidia.
The forth micro-shot is of spores from the gill at 1000x in congo red. The ave. spore size is : length – 7.16 +/- 0.43 (err 0.17) um, width – 5.77 +/- 0.58 um (err: 0.22) – q : 1.25 +/- 0.11, on 8 spores.
With the single projections to the smaller cystidia, from Funga Nordica this matches – H. grisea. It seems some sources have Hohenbuehelia atrocaerulea var. grisea and other have Hohenbuehelia grisea. And Index Fungorum call these synonyms. So, anyway this seems to be a good id, even though others seem to find this id fairly questionable or uncertain.
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OK, I am feeling the pressure to return to my scope…;)
Ironically, IMO, those encrusted cystidia actually showed up better in the unstained version!
Hohenbuehelias have two types of cheilocystidia – those big thick-walled ones with the crystals which take front page, but also, small one with 2 or 3 finger like appendages. you have to squash the slide a bit to get to see those properly
I’ll use congo red on my examples of this one from San Diego.
Well, there are a number of reasons. First perhaps, is that I wanted to see that the spores were hyaline, so I could tell that these weren’t Crepidotus with some tan or pigmented spores, and congo red could obscure that. Also I had been looking at Psathyrella and Inocybe lately (boy the life I lead!), and I wanted to be able to see if the cystidia walls were pigmented. And in the end, because I just hate to use congo red, I just do…
Now that I know that there are no pigmentation to look for at all, which I didn’t before hand, using congo red seems obvious. And I should try to use it in the future, if I get back to looking at these… which will probably never happen. And I’ll still hate to use congo red…
Very cool stuff. Why not use a drop of a colorizer on the micro shot? Congo Red works great in such cases..D.
Created: 2010-02-28 06:12:02 PST (-0800)
Last modified: 2010-02-28 06:12:02 PST (-0800)
Viewed: 393 times, last viewed: 2018-05-30 10:40:20 PDT (-0700)