Observation 48067: Lichen P. Micheli
When: 2010-06-30
Who: zaca
No herbarium specimen

Notes: Very similar in shape to Ionaspis lacustris (see here), the color being different.

Proposed Names

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Eye3 Eyes3
Recognized by sight

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Eye3 = Observer’s choice
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Comments

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microscope
By: Jason Hollinger (jason)
2010-07-11 09:48:02 PDT (-0700)

I’ve known professionals who get by with 500×. If it has good quality resolution, it’s probably better than mine even at 1000x!

However, I highly recommend investing in a dissecting scope. It is one of my most prized possessions. Useful for so many things, even beyond lichenology and botany. Quality is less critical, so even the cheap one I bought on Ebay has performed admirably:

10x-30x stereo dissecting scope ($112)

Thanks, Jason, for the very detailed instructions.
By: zaca
2010-07-10 18:12:32 PDT (-0700)

I have no dissecting microscope, so I have to do it with a head magnifier. Also I don’t have a personal microscope, but sporadically I can use one with the maximum of magnification 500×. I hope it be enough. As a matter of fact, first I have to find the specimen again.

apothecial section
By: Jason Hollinger (jason)
2010-07-10 17:33:18 PDT (-0700)

Ideally you could place a few vertical slices of the apothecia under a microscope. Best way seems to be to place a specimen under a dissecting microscope, carefully slice one of the apothecia with a very sharp razor, trying to make the slices as thin as possible. There are various tricks involving thumbnails and other spacers, but it can also be done simply with a steady hand. My method is to make one cut near the left side, say, and clear away the edge on that side. Then make a few vertical cuts as thin as possible on that side. Cut them free from the substrate with a horizontal cut (very carefully so they don’t go flying off!) Place a drop of water on a slide, dab the edge of the razor in the drop, then touch the razor to the tiny slices. The water should cause them to stick to the razor’s edge. Now dab the razor back in the drop of water and it should release the slices. Now place a cover slip over them, squash gently if desired, and place under microscope. You will need at least 400x to see spores, better yet 1000×. It can be difficult to tell whether you’re just looking at a young ascus with rough-textured contents, or a mature ascus filled with hundreds of tiny 2 µm spores, especially at 400×. But if it’s Aspicilia or Ionaspis you should be able to see the mature spores pretty well even at 400×. If Acarospora, check for an ascus that has burst open spilling the tiny spores into the water surrounding it.

What kind of analysis should be performed?
By: zaca
2010-07-10 16:06:39 PDT (-0700)

I suppose that the first step is to look at the asci and spores. How to make such preparation? If “dozens to hundreds” of tiny spores per ascus confirms, how to proceed?

By: Jason Hollinger (jason)
2010-07-04 10:51:27 PDT (-0700)

That’s one of the other genera with immersed apothecia. Acarospora has “dozens to hundreds” of tiny spores per ascus. No other “polysporous” genera have immersed apothecia except Pleopsidium, but that one is bright yellow.

Created: 2010-07-04 10:41:19 PDT (-0700)
Last modified: 2010-08-14 11:38:43 PDT (-0700)
Viewed: 51 times, last viewed: 2016-10-26 15:36:33 PDT (-0700)
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