Observation 48725: Pluteus sect. Pluteus
When: 2010-07-16
Herbarium specimen reported

Notes: These were found growing in the EXACT same square foot as 46573, and are very similar, except for that 46573 were much lighter in color. Maybe the lot of them is P. magnus? First two pics are on first day after they emerged. Third pic is during second day, and cap surfaces have become lighter in color.

Spores at 400x in KOH.

Proposed Names

51% (3)
Eye3
Recognized by sight: Compare with 46573. See notes.
55% (1)
Recognized by sight: one thin cystidia and one larger one hmmm.
75% (1)
Eyes3
Recognized by sight

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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Hi Fredo.
By: Dave W (Dave W)
2015-04-24 16:42:34 PDT (-0700)

I’ll look through my collections for this material.

I think you did it here…
By: Douglas Smith (douglas)
2010-07-20 09:21:05 PDT (-0700)

Actually that looks pretty good really. I wouldn’t worry about getting the pointer out of the image, I’m not sure the image could get any sharper.

The cystidia pretty much tells you what you want there. The narrow one shows a couple pointed hooks on the end there, it isn’t sharply pointed. So this knocks out Pluteus magnus and some similar. This moves it towards Pluteus cervinus and many others.

If you can’t see the cystidia well enough, you can always squash the mount to seperate the cells, and this will seperate the spores also. Just wrap your finger in a tissue (to keep things cleaner), and push down gently on the cover slip, and rock your finger back and forth.

The horned cystidium
By: Dave W (Dave W)
2010-07-20 08:53:56 PDT (-0700)

in the photo certainly looks like the ones seen for P. cervinus on the Mushroom Expert page. I can find no info on cystidia for P. magnus, but I assume that the cystidia photo has provided someone with enough info to rule out magnus.

Still wondering about 46573 (which occurred in the exact same spot as the ones in this obs). Seems unlikely, though, that those would have been magnus.

Gills were completely covered in spores.
By: Dave W (Dave W)
2010-07-19 19:54:51 PDT (-0700)

But I did find one nice cystidium on the edge. Two pics are using different resolutions. At 400x in Meltzer’s.

Last remaining fruit body did get a bit soaked by a shower this morning, but I think it may be salvageable. I’ll air dry first (in the south facing attic window) and then finish off in the dryer.

At some point in time I’ll see if I can clean up the view on this scope… and maybe get that pointer removed from view… or made less prominent. Not enough hours in the day, you know.

your welcome
By: Johannes Harnisch (Johann Harnisch)
2010-07-19 09:43:45 PDT (-0700)

I finally did just that this year as well (venture in to the world of cystidia) but like i mentioned you may need to remove the measuring apparatus all to gather your eye piece should be easily removed and unscrewed in side of which is the eye piece graticule, because there is also dust it appears on the clear part of it (the measuring apparatus), also wipe any dust of the eye piece.
Very Best and Good Luck!
ps Really looking forward to the Cystidia Pictures!

Thanks Johann and Douglas
By: Dave W (Dave W)
2010-07-19 09:15:05 PDT (-0700)

for all the information. I think it’s time for me to finally venture into the mysterious world of cystidia.

Just called my wife, and she will harvest the one remaining Pluteus specimen. Hopefully, this morning’s light rainfall hasn’t caused the fruit body to lapse into a state of rapid decay. If not, I’ll dehydrate it in my homemade dryer, label, and store the specimen. If I have time tonight, I’ll search for the cystidia. I’ll try KOH, and if things look ill defined, I’ll try mounting in Meltzer’s.

That’s a nice point about the measuring apparatus possibly throwing off my camera focus through the scope. I’ll try to find a way to move the pointer out of view.

Spores were collected by taking a print directly on a slide. I forgot to straddle the slide with the mushroom cap, so the very thick print covered the entire slide, placing too many spores into the scope view.

Oh one more thing
By: Johannes Harnisch (Johann Harnisch)
2010-07-19 07:50:58 PDT (-0700)

when you take pictures through the scope I would suggest that you take some with and some with out your eye piece graticule(the measurement thing with that needle) what is happening is that your camera is focusing on the dust and the needle of it and you are not getting as sharp a picture of the fungal tissue, you may be able to correct that some with a larger F stop but i would still suggest that you remove the graticule for at least some pictures. :D

yes I find it very interesting to keep a dried specimen
By: Johannes Harnisch (Johann Harnisch)
2010-07-19 07:37:30 PDT (-0700)

I really recommend it…also you don’t have to wait for the specimen to dry before you can take a look at the gills under the microscope, just take a gill of it and have a look.

Oh, yeah…
By: Douglas Smith (douglas)
2010-07-19 05:05:12 PDT (-0700)

Yeah that is fine, using 400x for cystidia. If you look at my photos of cystidia that I’ve posted here (which are quite a few at this point), they are all at 400×. Mounting in KOH is fine, even water really. I fine KOH is better since it breaks up the surface tension and you don’t get air bubbles clinging to the gill edges like you can with water… although tapping the slide a few times helps with that. Some people complain about clear reagents to look at clear cystidia, but tell them to get over it. Stained slides could be better, but KOH is fine (or water, tapping the slide…).

Use the gill whole, at least I do, so you can see where on the gill the cystidia are attached. To tell if they are edge cystidia or facial cystidia. Or you could just squash it to mix up all the cells, so you could see cystidia to the base.

But it is fine, just pluck a part of a gill, getting a bit of the edge, and lay it down on the slide. Add drop of water or KOH, add cover slip, tap gently a couple times, and put it in the scope.

For mushroom getting old, you can just dry them out completely, with a food drier or such, and then put them in a zip-lock bag to keep them around longer. Hopefully with a small label so you know what they are also… then you can pluck off a gill with fine tweezers months later, and check on the cystidia. (Which I’m doing now, finally for stuff I got last month…)

From where on the fruit body did you get the spores?

Is 400x enough magnification
By: Dave W (Dave W)
2010-07-19 04:48:42 PDT (-0700)

to see any significant detail on cystidia? I haven’t done this sort of thing. Does one mount the gill material in KOH? Does one chop up the gill material? there’s still one of these on my lawn… although it’s beginning to dry out.

Spores were obtained from one of the fruit bodies in the photo.

Photo of cystidia?
By: Douglas Smith (douglas)
2010-07-19 02:34:54 PDT (-0700)

You have a scope there, where did you get spores for the shot? You should put a gill under the scope and get a shot of the cystidia perhaps, that would be interesting.

We really!
By: Johannes Harnisch (Johann Harnisch)
2010-07-18 18:14:09 PDT (-0700)

need some microscopic info on the Cystidia of these in order to vote correctly,

Created: 2010-07-18 18:03:23 PDT (-0700)
Last modified: 2015-04-24 13:13:08 PDT (-0700)
Viewed: 129 times, last viewed: 2016-03-24 01:48:09 PDT (-0700)
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