Observation 52169: Amanita chrysoblema G.F. Atk.
When: 2010-09-06
No herbarium specimen

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Wow, interesting!
By: Eric Smith (esmith)
2011-08-17 18:21:53 CDT (-0400)

Rod, thanks so much for your ID help.

material received …
By: R. E. Tulloss (ret)
2011-08-17 15:29:53 CDT (-0400)

I checked the spores with Melzer’s Reagent and they are inamyloid.

I would say that this may be a specimen of the white color variant of A. amerimuscaria.

R.

Could this be the same critter as …
By: Paul Derbyshire (Twizzler)
2010-09-08 13:05:50 CDT (-0400)
Ah…
By: R. E. Tulloss (ret)
2010-09-08 00:02:09 CDT (-0400)

It’s the spores that matter. If they didn’t change color, then sect. Amanita is the likely home of this critter. Look for a place where you can compare the spores to a background of hymenial surface. Sometimes, this can convince you whether or not you are seeing an amyloid reaction in the spores…because the basidia don’t change in Amanita (except for two known cases in which the contents of the basidia become reddish in iodine).

Another trick is to look for a cluster of spores and see if the regions in which the spores overlap are significantly darkened (indicating that they are all reacting somewhat to the iodine).

A third trick is to compare spores to air bubbles. An amyloid spore should be darker than an adjacent air bubble. An inamyloid spore should be about the same shade as a nearby air bubble (when both are in iodine solution).

A fourth trick is to see if you can see the contents of mature spores. You usually will not be able to do that with amyloid spores. If you can consistently see into mature spores, then they are inamyloid. There is a vague middle ground with this trick. Some inamyloid spores become semiopaque in Melzer’s but don’t turn color…and, hence, aren’t amyloid spores.

Hope this helps.

Very best,

Rod

Highschool surplus…400x
By: Eric Smith (esmith)
2010-09-07 23:59:36 CDT (-0400)

It’s not much but it’s better than nothing. I’m not much of a microscopist.

I removed a single gill and mounted it in a lot of iodine. I observed a few vivid blue and brown feature (contaminates?) but the spore didn’t seem to change color.

These mushrooms didn’t have that sticky floccose material that sticks to your fingers.

That is interesting…
By: R. E. Tulloss (ret)
2010-09-07 23:55:29 CDT (-0400)

So this could well belong in sect. Lepidella…as you propose. Interesting.

R.

Spores seem inamyloid
By: Eric Smith (esmith)
2010-09-07 23:22:45 CDT (-0400)

.

By the time..
By: Eric Smith (esmith)
2010-09-07 21:38:31 CDT (-0400)

I tried to take a spore print the material had been drying for several hours and I failed to get a print. Is there another method?

I think so.
By: R. E. Tulloss (ret)
2010-09-07 00:42:45 CDT (-0400)

Remember not to make the test on a piece of paper, would, cardboard, etc. Those substance will react with the iodine and you may not get to see what you want to see (i.e., a pile of spores becoming quite dark instead of just being the same color as the solution.

Do it on a piece of glass or a piece of white china or a white porcelain surface.

Plastics might react with the solvent of the tincture.

Very best,

Rod

Will iodine work?
By: Eric Smith (esmith)
2010-09-06 21:28:32 CDT (-0400)

10% tincture?

Can you check amyloidity of spores?
By: R. E. Tulloss (ret)
2010-09-06 21:13:56 CDT (-0400)

Just to make sure this isn’t a sp. of sect. Lepidella.

R.

Created: 2010-09-06 16:51:02 CDT (-0400)
Last modified: 2014-03-20 15:57:29 CDT (-0400)
Viewed: 234 times, last viewed: 2016-10-25 13:18:22 CDT (-0400)
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