Observation 5386: Inocybe brunnescens Earle
When: 2007-11-25
Herbarium specimen reported

Notes: Uploading some microscopic work.

These were found in the duff under live oak. Getting around to looking at Inocybe before the new season starts up here.

The first micro-shot is of the gill edge at 400x in Meltzer’s. Here the cheilocystidia can be obs, which are long and clavate, with broad blunt ends. No pleurocystidia were obs. in these.

The second micro-shot is of the stipe surface near the apex at 400x in KOH. Here lots of caulocystidia can be obs., dense and common, and similar to the cheilocystidia. No caulocystidia were obs in the bottom half of the stipe.

The third micro-shot is of the spores at 1000x in KOH, from the stipe apex. The spores are ellipsoid, blunt, smooth, and very light tan, without a germ pore or nodules. The ave spore size : length – 8.62 +/- 0.54 (err 0.14) um, width – 5.40 +/- 0.28 (err: 0.09) um – q : 1.60 +/- 0.08, on 16 spores.

Putting this together with F. Nishida’s key to California Inocybes, along with the univ. veil tissue embedded in the cap surface, this comes out as Inocybe brunnescens.

As a bonus, when I was looking for cystidia in the bottom of the stipe, I found these funky guys, and this is included in the forth micro-shot. These things were like warted globes on long thick hyphae (without clamp connections…), and were found on the surface of the stipe. They were quite common, but I’m pretty sure they are not part of the Inocybe. What they are, I have no idea, but they are cute, and pretty funky. If anyone has an idea, post it!

Proposed Names

86% (1)
Eye3 Eyes3
Used references: Nishida, F.H. (1989). Key to the Species of Inocybe in California
Based on microscopic features: Cheilocystidia clavate, pleurocystidia lacking, spores ellipsiod and smooth, veil tissue on disc of cap.

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Eye3 = Observer’s choice
Eyes3 = Current consensus


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Created: 2007-12-09 22:47:39 CST (-0500)
Viewed: 41 times, last viewed: 2016-10-22 21:43:37 CDT (-0400)