Observation 60773: Galerina Earle
When: 2010-11-29
Herbarium specimen reported

Notes: 11-29-10
Rowan Co., NC
USA

Pileus:
1.6cm broad, convex with nipple, smooth, striate margin(.5cm)
Brown

Lamellae:
Adnexed, subdistant, cinnamon-brown

Stipe:
6cm long, .2cm wide, smooth, equal, hollow
Concolorous with pileus, turning darker brown on lower half

Smell:
Faintly Fungoid

Spore Print:
Yellow-brown

Spores:
Elliptic, smooth

Images

126525
Copyright © 2010 Matt
126526
Copyright © 2010 Matt
126527
Copyright © 2010 Matt
131232
800x in water
131233
2000x in water
136581
Cheilocystidia 800x in Melzer’s
136582
Cheilocystidia 2000x in Melzer’s
136583
Cross section 200x in Melzer’s – showing lamellar trama
136584
Lamellar trama 800x in Melzer’s
136585
Pileipellis 800x in Melzer’s

Proposed Names

64% (3)
Eyes3
Recognized by sight
28% (1)
Recognized by sight: Little, brown
Based on microscopic features: Spores smooth
28% (1)
Recognized by sight: Little, brown
Based on microscopic features: Spores smooth

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

Add Comment
Stains
By: Matt Sherman (Shermanii)
2011-03-08 17:58:31 PST (-0800)

Thanks for the stain suggestions Alan!

stains
By: Alan Rockefeller (Alan Rockefeller)
2011-03-08 17:38:51 PST (-0800)

I favor cotton blue at the moment, but congo red can be ok too. Often the congo red solutions are too dilute.

Stains
By: Matt Sherman (Shermanii)
2011-03-08 12:50:49 PST (-0800)

I don’t have any stains at the moment.

Could you suggest a few stains for me to look into?

Sorry about the crap pictures. I’ll check this one out again once I get some stains then I’ll take some better photos.

cystidia
By: Alan Rockefeller (Alan Rockefeller)
2011-03-08 06:05:04 PST (-0800)

I can not see the shapes of the cystidia very well. Perhaps try a stain or stained crush mount.

Microscopy added…
By: Matt Sherman (Shermanii)
2011-03-07 23:04:35 PST (-0800)

Check ‘em out if you’d like…

Some of these photos are not that great. My apologies.

water
By: Matt Sherman (Shermanii)
2011-01-18 12:14:53 PST (-0800)

Thank you for this information Douglas.

I only have one specimen, so I’ll try experimenting on other mushrooms first. Then I’ll check this one out and post what I find later.

Once again…Thank you guys very much for helping me out!

Rehydrate
By: Douglas Smith (douglas)
2011-01-18 10:04:29 PST (-0800)

For the dried material, I after getting the section, I put the dried material on the slide, and hit it with a drop of alcohol, and then let that go away. This get the rehydrating going and also kills bacteria on the sample. So then your spores will stay still, and not get bumped around at high mag. You can use water, but make sure you tap the slide a good few times to dislodge air bubbles trapped against the material. With water’s high surface tension this is all too easy, and can make it hard to see the fine cystidia. KOH won’t trap air bubbles so easily.

Also for cystidia, since they usually have clear cell walls, people like to use a stain like Congo Red. I don’t like to do that, some many people do.

But you can do it with water just fine, just tap the air bubbles away.

KOH?
By: Matt Sherman (Shermanii)
2011-01-18 09:27:10 PST (-0800)

Thanks for explaining that Alan.

Do I have to use KOH for rehydrated specimens?
Or can I use water?

spores
By: Alan Rockefeller (Alan Rockefeller)
2011-01-17 17:37:05 PST (-0800)

While Galerina sometimes has spores that are smooth or so slightly roughened that they look smooth in pictures, Douglas is probably right that these are not Galerina spores. Other genera to look at include Tubaria and Conocybe. You can check the pileipellis, cheilocystidia and pleurocystidia all at once if you hold a fresh cap in your hand, cut off about 1 mm from the edge, then make another parallel cut .1 mm in. Make it on an angle so half the section is thicker and the other half is extremely thin, then throw away everything that is not extremely thin and mount the thin part in water. To establish the genus you will need to take a good look at the pileipellis structure and compare with the drawings in a mycological glossary.

Genus
By: Matt Sherman (Shermanii)
2011-01-17 08:48:07 PST (-0800)

Is there another genus that comes to mind?

When ever I purchase an ocular micrometer, I’ll post the spore size.

What would I be looking for on the cap’s surface?

About the cystidia. I’ll have to play around with that under the microscope. I’m not very experienced yet, but I’ll see what I can do.

Not really Galerina spores…
By: Douglas Smith (douglas)
2011-01-16 10:31:36 PST (-0800)

Those spores are rather smooth, and not really Galerina spores. It might be something related to G. sideroides, it would be good to know the size of the spores on that one. But it might not be a Galerina at all. Need more info, like the cap surface, and the gill edge cystidia at least.

Created: 2010-12-14 08:56:09 PST (-0800)
Last modified: 2011-03-07 23:00:30 PST (-0800)
Viewed: 199 times, last viewed: 2016-03-29 09:28:40 PDT (-0700)
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