Observation 69366: Amanita Pers. subgenus Amanita
When: 2011-06-15
Herbarium specimen reported

Notes: These were rather small with caps up to 3.7 cm across.
They were growing in the middle of a dirt road among mixed conifers.
Couldn’t get a decent spore print so I washed a gill with some Congo Red. I only measured a few spores but they were in the range of 9.2-12.0 X 7.1-9.0 microns and the Q values were pretty consistent, in the range of 1.23-1.38(ave. 1.31).

Images

151711
151712
151713
Spores in Congo Red @ 1000X
151714
Spores in Congo Red @ 1000x
151715
Spore in I-KI solution @1000X
152796
Universal veil from basal volva in Congo Red @ 1000X.
152826
Universal veil from basal volva in Congo Red @ 1000X.

Proposed Names

57% (1)
Recognized by sight
86% (1)
Eye3 Eyes3
Recognized by sight

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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hold on now…
By: Debbie Viess (amanitarita)
2012-05-21 11:23:04 CDT (-0400)

w/out having the mushroom in hand (or having a chance to do the work), or having somebody run the DNA (spore amyloidity woulda told us the section right off, however, DNA be damned) we are conjecturing, correct?

So, no mea culpas necessary. We change our ID theories to fit new and better information, but we gotta start somewhere. ;)

Your vast experience and feedback is ALWAYS appreciated here, Rod.

EVERYBODY makes a wrong guess sometimes. It’s no big deal. In fact, we all made the same wrong first guess here, originally.

Being able to see contents in an amyloid spore …
By: R. E. Tulloss (ret)
2012-05-19 16:44:06 CDT (-0400)

would be very unusual. I made a mistake.

R

Not a Lepidella
By: Dimitar Bojantchev (dimitar)
2012-05-19 14:42:59 CDT (-0400)

I know this Amanita, Ron just drew my attention on MushroomTalk. This is one is in subg. Amanita. I have the phylo position pretty well nailed. Fairly well spread species under both conifers and oaks, but not too common. Can be found in suburbia and in the woods, but mostly late winter and spring. I did think Lepidella at first though…

Dimitar
Dried materials have arrived.
By: R. E. Tulloss (ret)
2011-06-24 21:41:48 CDT (-0400)

They’re in cool, dry storage after their trip through summer humidity. I hope to have a look at them soon.

Very best,

Rod

You’re welcome.
By: R. E. Tulloss (ret)
2011-06-20 21:00:29 CDT (-0400)

When air bubbles are so plentiful that they block your view, or even if they are just an annoyance, you can reduce the surface tension of the air bubbles and cause them to coalesce by adding a drop or two of alcohol (regular rubbing alcohol will do) at an edge of the cover slip closest to the offending bubbles. You can draw the alocohol under the cover slip by wicking away liquid from under the coverslip on the side of the cover slip opposite the one where you placed the drop of alochol. You can experiment with wetting the material before you make the slide [wet it with alcohol or get it moist in a humid chamber (closed cup, e.g.)] before you make a section and cover it for a clide.

Very best,

Rod

Thanks Rod,
By: Ron Pastorino (Ronpast)
2011-06-20 18:31:04 CDT (-0400)

I’ve replaced that photo with another from the same section.
Also, will be sending specimens.

first of limb images
By: R. E. Tulloss (ret)
2011-06-20 17:46:56 CDT (-0400)

The first image definitely suggests that the upper edge of the limb is composed largely of filamentous hyphae. The second (lower) image looks as thought the view is blocked by an irregular air bubble or a confluence of bubbles that is/are distorting the image of what is (probably) behind it/them.

I’d, of course, be glad to look at a sample of the material: Minimally, A fruiting with clearly defined volva on cap and bulb and (if different) a fruiting body likely to be mature with sporulation well underway.

Very best,

Rod

Added some micro photos of universa veil
By: Ron Pastorino (Ronpast)
2011-06-20 17:16:54 CDT (-0400)

which i guess would be called filamentous to my untrained eye.
Will gladly yield material to more experienced and interested party(s).

I’m Melzerless in Marin
By: Ron Pastorino (Ronpast)
2011-06-20 14:56:57 CDT (-0400)

and the I-KI solution is not always reliable. However, as Christian noted, the few spores that I could get did seem relatively dark under the microscope.
I’ll try to do a little more microscope work on them in the next day or two.
Ron

Thanks, Christian.
By: R. E. Tulloss (ret)
2011-06-20 14:50:14 CDT (-0400)

Good eye.

Then let’s continue to suppose its a lepidella.

Take a look at the top of the bulb of the top left fruiting body in the first image and in the leftmost fruiting body (as well as others) in the second iamge. I think I see a short and thin, but very distinct, mebranous limb standing up from the bulb at about the point at which the outer part of the cap probably touched the bulb in the “button” stage. This would really move things forward if other people agree that it’s not a trick of aging eyes.

This would really cut down the possibilities taxonomically for our little friend. A very short, thin, limbate volva in section Lepidella indicates a very thin, mostly hyphal layer on the outside of the volva. The limb would have a powdery inner surface representing the inner part of the volva. This sort of volva is present in subsection Limbatulae (“little limbs”) and ecologically is very often associated with Mediterranean and/or “leaky” ecosystems. A little limb is known to occur less frequently or in different form in some other lepidellas. I think we can at least work this through to a point below the subsectional level. Bas emphasized three characters for coarser levels of Lepidella taxonomy: volval anatomy, clamps, and spore size/shape. We might be able to deal with this through Ron’s photoskills. :-)

R.

Rod

if amyloid…
By: Debbie Viess (amanitarita)
2011-06-20 13:07:37 CDT (-0400)

I’d go with lepidella too.
nice find, Ron.

I see blue tones
By: Christian (Christian Schwarz)
2011-06-20 10:16:58 CDT (-0400)

in the last spore photo (in Iodine solution), so I’d say they are amyloid.

After some jogging of memory…
By: R. E. Tulloss (ret)
2011-06-20 10:15:31 CDT (-0400)

David Biek wrote a little book of California mushrooms a couple of decades back (at least) and he had two unnamed (or unidentified) taxa called “Little White” and “Castle Crags White” mentioned in the book. I think they had marginate bulbs? I now think one of them (of which he sent me some material in those ancient days) may have been A. silvicola.

Your spore size range is on the large size for sivicola.

A quick check of checklists organized by spore size and shape did not yield anything known from California and fitting your spore data.

I’ve been going under the impression that your material belongs in subsection Solitariae of section Lepidella. If you could check the spores in Melzer’s Reagent, that would reduce the unknowns. Also, if you can provide a picture of the universal veil under the microscope, we can see if I’m really focusing in the right place. Clamps on basidia: present or lacking? That would also help narrow things down

Thanks for emaling me about this post, Ron…
By: R. E. Tulloss (ret)
2011-06-20 09:25:03 CDT (-0400)

Can you tell me if the spores are amyloid?

R.

Created: 2011-06-17 00:32:41 CDT (-0400)
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