Observation 80177: Mycena oregonensis A.H. Sm.
When: 2011-10-19
Herbarium specimen reported

Notes: Spores 5-6 × 3-3.5 microns, apparently inamyloid.

Proposed Names

28% (1)
Eye3
Recognized by sight
77% (2)
Eyes3
Recognized by sight

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Eye3 = Observer’s choice
Eyes3 = Current consensus

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focus
By: Sava Krstic (sava)
2011-10-21 22:04:15 PDT (-0700)

Alan, this is all new to me. Thanks again. Now I need to experiment a little to see how it works.

focus
By: Alan Rockefeller (Alan Rockefeller)
2011-10-21 18:11:55 PDT (-0700)

No matter how you set the focus on the camera, you can get the subject to be in focus by adjusting the fine focus on the microscope. The focus on the camera affects the sharpness of the measuring scale in your reticle. To get everything as clear as possible its best to use a camera with manual focus because the automatic focus doesn’t always focus onto the reticle. Sometimes it does though, in which case the measuring grid is razor sharp. Last week I tried taking a dozen pictures at different focus levels and found that the grid was sharpest if I set the manual focus to about one meter. Other setups might have different distances. I have gotten good results with automatic focus cameras however you don’t have much control over the sharpness of the scale (unless you press the shutter half way, focus on something one meter away, put it to the eyepiece and press the shutter the rest of the way). Let me know if you have any questions.

Focusing problem
By: Sava Krstic (sava)
2011-10-21 17:01:27 PDT (-0700)

Alan, thanks for trying to help. Taking microshots frustrates me a lot and I am not happy with what I’m getting. Now, sorry to say, I don’t quite understand your instructions. I’m taking these shots with my old Canon A2000IS in the macro mode. This camera does not have manual focus, but I can also use my G12, which does. It’s not clear to me how can setting manual focus to an approximate value (1m) get things in focus. If you can explain a little more, I’d greatly appreciate it.

focus
By: Alan Rockefeller (Alan Rockefeller)
2011-10-21 15:50:46 PDT (-0700)

I notice that in the micrograph, the micrometer is out of focus. In my setup I fix this by setting the manual focus to about 1 meter. The pic is also a bit grainy, indicating that the ISO is probably set too high. On many cameras you can set the maximum ISO. Its a good thing to set at around 800 or so, maybe less depending on your camera, so it doesn’t automatically set it to something that gives you really grainy photos.

Thanks, Douglas
By: Sava Krstic (sava)
2011-10-21 00:29:05 PDT (-0700)

Pleurocystidia match; spore size not quite. I’ll try to check some other microcharacteristics when I get a chance.

Created: 2011-10-20 23:05:59 PDT (-0700)
Last modified: 2011-10-21 15:48:13 PDT (-0700)
Viewed: 518 times, last viewed: 2016-10-25 07:59:29 PDT (-0700)
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