Notes: I crushed one of the tiny apothecia to see if I could observe the spores. Just got to see an ascus with 8 spores of ellipsoidal shape and approximate dimensions of 10.5 × 5.5 µm clearly 1-septate.
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In this case my concern more than the septation of spores that now I know very well that can be apparent it is the size of the spores. But, I have no better proposal and I do not pretend to be a sp. nov.
will responde to the question of the dimensions of the ascii, but doesn’t responde to: (1) the dimensions of spores – I don’t know any Ochrolechia with spores less than 20um (maybe more) in lenght; (2) the existence of some 1-septate spores (on the left of the last picture attached there are some).
Concerning your question: The blue-tipped ascii were stained in Melzer’s solution.
Thanks, Jason, for the interest.
Something about the large asci and spores suggests that genus. Interesting. This specimen shouldn’t have been hard to put in a genus, at least. Obviously more bizarre lichens out there than I’ve dreamed of! Are the blue-tipped asci stained in Lugol’s or Melzer’s solution?
I tryed again to have more information from microscopy, this time with more success (see attached photos). This new attempt was clearly dominated by the observation of the ascii which are very big (in one of the photos there is one with almost 300 µm of lenght). Concerning the spores, some are 1-septate and others is not possible to see any septation (at least with my equipment). The values for their dimensions obtained were much bigger than with the small number observed previously:
Me = 15.8 × 9.8 µm ; Qe = 1.6 (N=45).
I read the generic description of all genera mentioned by Jason in her message and none have clear cylindrical ascii – some are ± clavate and others clavate to cylindrical – though some species might have.
To complete the information, let me mention the chemical reactions on thallus: C-, K+y, KC+y.
Crusts with thalline apothecial rim and colorless 1-septate spores on either bark or rock are often Lecania. Micarea, Biatora, Mycobilimbia and others also have hyaline 1- to many-septate spores, but none have apothecia with thick margin with algae in it. Another to consider is Haematomma, although that genus usually has bright red apothecia which react strongly K+ violet or red. But it, too, has thalline rims and colorless, septate spores.
Created: 2011-10-25 06:34:04 AEST (+1000)
Last modified: 2011-10-25 06:34:05 AEST (+1000)
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