Observation 83484: Galerina minima (Peck) A.H. Sm. & Singer
When: 2011-11-20
Who: Byrain
Herbarium specimen reported

Notes: Growing on moss, they have an enlarged stem base and don’t appear to have a veil.

This observation has slightly roughened dextrinoid spores without a germ pore and slightly thickened walls, I believe they have a smooth plage. Based on 30 measurements the range is 8 – 10 × 5 – 6 μ, the average size is 8.81 × 5.75 μ, and the average Q is 1.53. The pileipellis has clamp connections and the basidia are 4-spored.
Cheilocystidia measurements – 34×10, 45×11, 45×17, 46×11, 54×11
Pleurocystidia measurements – 59×13, 74×14

The cheilocystidia and pleurocystidia pictures are too blue, I tried to correct them with gimp, but I think they were too far gone to fix completely.

Images

185732
185733
185734
185735
Dextrinoid slightly roughened spores
1000x
Mounted in melzers
1μ divisions
185736
Cheilocystidia
1000x
Mounted in melzers
1μ divisions
185737
Pleurocystidia
185738
Pileipellis with clamp connections
1000x
Mounted in melzers
1μ divisions
185739
4-spored basidium
1000x
Mounted in melzers
1μ divisions
185773
Caulocystidia
1000x
Mounted in KOH
1μ divisions
185774
Spores on stipe
1000x
Mounted in KOH
1μ divisions
185818
November 27th
Has a hygrophanous pileus.

Proposed Names

57% (1)
Recognized by sight
51% (3)
Recognized by sight
Based on microscopic features: All looks pretty good.
0% (2)
Recognized by sight: Closest ITS match – only 2 base pairs away when the differences due to ambiguous bases in the reference sequence are subtracted. Interestingly, the closest match was collected in Greenland, and the majority of G. atkinsoniana sequences in GenBank match more like 97%. The top match is likely misnamed. Another sequence from Greenland, labeled G. minima matches just as well.
85% (1)
Eye3 Eyes3
Recognized by sight: Pileus not innately fibrillose with a straight (Not incurved) margin. Scattered veil remnants on the stipe that possibly form a distinct annular zone on some specimens. On mosses and soil known from New York, Alabama, Idaho, Washington and Oregon.
Used references: A Monograph of the Genus Galerina Earle – Smith & Singer (1964)
https://blast.ncbi.nlm.nih.gov/Blast.cgi#alnHdr_51037609
Based on microscopic features: Clamp connections present, non-calyptrate roughened spores (8-10 µm) that have a distinct smooth plage and thin-walled pleurocystidia with an acute apex.

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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ITS sequence
By: Alan Rockefeller (Alan Rockefeller)
2016-06-24 16:26:12 CDT (-0500)

atgaACTTGatGTGGTTGTAGCTGGTCCTCTTGGGGACATGTGCACGCCCGTCATCTTTATCTTTCCACCTGTGCACCCTTTGTAGACCTGAATAGTACTTTCCGAGGCAACTCGGTCGGGAGGACTGCTGTGTGTTAAGCCAGCTTTCCTTGCATTGTTCAGGCCTATGTTTTTCACATACCCCTTAAAATGTAACAGAATGTATTATTTGGCCTTGTGCCTATAAACTATATACAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTAAATTCTCAACCTTACCAGCTTTTGCGAGTTGATAATGGCTTGGATGTGGGGGTCTTTTGCTGGCTTCATCAGAGGTCTGCTCCCCTTAAATGCATTAGCCGGTGCCCTGCGTGGAGCCGTCTATTGGTGTGATAATTATCTACGCCGTGGACGTCCACTTTTAATGGGATGCGCTGCTTCTAACCGTCCATTCACTTGGACAATTAATGACAATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAAGCATATCAata

Douglas
By: Byrain
2011-11-27 23:21:40 CST (-0600)

Thanks for the id, I have a pdf of “A Monograph of the Genus Galerina Earle” and unless its under another name I don’t think G. vitteformis is in here. Do you have any literature suggestions?

Edit: I added another image with a ruler showing the hygrophanous pileus.

Created: 2011-11-27 18:21:48 CST (-0600)
Last modified: 2016-06-26 19:49:01 CDT (-0500)
Viewed: 195 times, last viewed: 2016-10-22 21:49:02 CDT (-0500)
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