Observation 84441: Lichen P. Micheli
When: 2011-12-10
Who: Byrain
No herbarium specimen

Notes: On exposed small roots at the base of a Washingtonia sp. (W. filifera or W. robusta), these are very small and hard to see with the naked eye. The texture is a lot harder then they look and with a little effort I managed to scrap a little bit onto a slide and found interesting stuff…

Images

188800
188801
188802
100x
Mounted in KOH
188803
Spores?
188804
188805
1000x
Mounted in KOH
1μ divisions
188950
100x
Mounted in KOH
188953
Ascus?
1000x
Mounted in KOH
188954
Spores
188955
Spores

Proposed Names

28% (1)
Eye3 Eyes3
Recognized by sight
Based on microscopic features

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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Those keys
By: Jason Hollinger (jason)
2011-12-12 02:03:37 EST (-0500)

They’re incredible, aren’t they?? Really wish someone else had taken up the torch when Bruce Ryan passed away 5-6 years ago. Still, great source, even if slowly becoming out-dated.

I know
By: Byrain
2011-12-11 20:39:55 EST (-0500)

but so many things to get and a limited number of funds, its definitely up there on the list though!

And those lichen keys are quite interesting and appreciated.

Dissecting scope is “must” for lichenology
By: Jason Hollinger (jason)
2011-12-11 19:47:47 EST (-0500)

Check eBay; you can get perfectly good ones for ~$100. Mine is 10x-30x from “precision*world”. I’m still very happy with it after ~5 years. Don’t know what I would’ve done without it. Don’t wait, it’s just not worth it! (Also useful for straightening out pins on CPUs in a pinch — that’ll pay for it twice over right there! :)

No dissecting scope yet.
By: Byrain
2011-12-11 19:12:38 EST (-0500)

I’ll see if I can use one at the local university.

Odd spores
By: Jason Hollinger (jason)
2011-12-11 18:59:42 EST (-0500)

In image 188953 the spores look multi-septate with lens-shaped lumina. Maybe you have some Graphis spores mixed in with the other weakly-septate Biatora-like spores. Contamination is always possible.

Do you have a dissecting scope? Granted, when the apothecia are this small, it’s difficult to get a clean apothecial section even with a dissecting scope and good sharp razor. Still, if you manage to slice one open, even if the section is too thick to work under the compound scope, you should be able to see through the dissecting scope whether the apothecium is pale inside or black.

More micro shots
By: Byrain
2011-12-11 18:49:48 EST (-0500)

I tried to do an apothecial section, but I can’t really see what I am cutting and I believe I failed…

Though, I manged to get some better spore shots and perhaps an ascus?

Spores are good, better to get a good apothecial section
By: Jason Hollinger (jason)
2011-12-11 01:41:46 EST (-0500)

For example, if the tissue below the apothecium is dark brown, it rules out Biatora. The key character for Micarea in the Sonoran Flora is:

Apothecia with poolr developed proper exciple, immarginate; paraphyses and excipular hyphae similar, abundantly branched and anastomosed; algae usually micareoid (less than 8 µm across, often in pairs, penetrated by intracellular haustoria); thallus often goniocyst-like

If that doesn’t match, you wind up at this couplet:

Ascospores partly simple; asci Biatora type . . . Biatora
Ascospores always 1-septate; asci Catillaria type . . . Catillaria or Catinaria

Look at Bruce Ryan’s unpublished keys for more information about all these genera:

pdf format
word perfect format

Good luck!

.
By: Byrain
2011-12-10 21:01:37 EST (-0500)

I’ll loook some more to see if I can find an ascus, are any spores or other things I found worth measuring?

Biatora, I think
By: Jason Hollinger (jason)
2011-12-10 20:50:48 EST (-0500)

The photos haven’t made it to the image server yet, so I can’t see full-size versions. (It’ll try again tonight automatically, sorry it takes so long sometimes.) The key feature is colorless septate elongate spores. According to the Sonoran Flora, that makes it Micarea, Biatora or Catillaria. Worth considering the other two genera, too. The keys require that you know the fine-scale structure of the ascus tip. Good luck! :) Chemistry might help, too.

Created: 2011-12-10 20:10:18 EST (-0500)
Last modified: 2011-12-10 20:10:20 EST (-0500)
Viewed: 88 times, last viewed: 2016-01-20 08:19:14 EST (-0500)
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