Observation 95678: Amanita “sp-S01” Tulloss
When: 2012-05-28
(35.5618° -84.2371° 259m)
Herbarium specimen reported

Notes: Banana yellow-green color of cap is reminiscent of A. citrina but does not have a veil.

Found on soil under hardwoods.

amyloid spores.

Proposed Names

28% (1)
Recognized by sight

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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Christine,
By: R. E. Tulloss (ret)
2012-05-29 21:22:25 CDT (-0400)

The information is for your use if you’re interested in exploring the material. I’d be interested in the results. But that’s secondary in terms of me posting the information a few minutes back.

R

The spores seen here
By: Christine Braaten (wintersbefore)
2012-05-29 21:05:51 CDT (-0400)

were taken from gill tissue near the margin. If you are interested, I can remeasure spores from a section taken near the stipe.

Spelling correct for species code number…
By: R. E. Tulloss (ret)
2012-05-29 21:03:55 CDT (-0400)

“Amanita sp. sp-1” should be “Amanita sp-S01” The “S” is the code letter I’ve used for years for “South Carolina” (first place where I collected this entity) and the zero before the “1” allows the website to present double-digit codes in correct numerical order.

Very bests

R

The spores suggest that the specimen
By: R. E. Tulloss (ret)
2012-05-29 20:59:19 CDT (-0400)

was immature or over mature. The average width is very close to that provided from 100 spores on the WAO site. Width is changes less than length does over the life of a fruiting body. Of course with a relatively stable width, the variable length directly corresponds to variability in the Q value.

If the specimen might have been on the young side when dried, I suggest measuring 10 spore from the stem end of the gills. This is where the mature of spores begins. If the specimen might have gotten a beyond prime condition before drying, there is a chance that spores would be most representative of the range seen in the species if you measure spores from a piece of gill from near the cap margin.

Good going so far. Good luck.

R

Micrographs showing spores in Melzer’s
By: Christine Braaten (wintersbefore)
2012-05-29 19:07:56 CDT (-0400)

Spores appear to be inamyloid, please see images added. Dimensions on spores in profile view: 7.95–8.88–9.3 ± 0.39 × 5.48–6.51–7.23 ± 0.51 μm, Q value 1.25–1.37–1.45 ± 0.072 (n= 10/1).

Compare color of spores in Melzer’s to color of air bubbles and color of basidia…
By: R. E. Tulloss (ret)
2012-05-29 09:07:03 CDT (-0400)

Melzer’s can spoil the transparency of spores sometimes, but not change the color of the spore walls. An air bubble won’t have changed color in Melzer’s neither will a basidium. You can also look for places where you have a “pile up” of spores in your mount. See if they become appreciably darker when they overlap each other. If so, this may be an argument for amyloidity. Looking for spores scattered on a gill surface (i.e., over a batch of basidia) will give you a chance to contrast the spore color to the color of the basidia.

Check out the basidium with four spores attached below and to the viewer’s left of the pointer in photo 222509. The basidium and the most mature spore seem to be the same color in that image. I agree with your idea of re-assessing the reaction to Melzer’s.

R

spores
By: AmatoxinApocalypse (AmatoxinApocalypse)
2012-05-28 23:12:45 CDT (-0400)

That is why I thought this was a good candidate for Amanita S01 but did not say it was, it sure looks like what others have said S01 looks like, but with fungi looks are often deceiving.

I look forward to seeing what you find Christine.

Amyloid spores
By: Christine Braaten (wintersbefore)
2012-05-28 22:26:17 CDT (-0400)

I will retest for amyloid spores again tomorrow and update with better micrographs. Thanks for all your interest in this observation!

if these are really amyloid spores…
By: Debbie Viess (amanitarita)
2012-05-28 22:02:00 CDT (-0400)

how can this amanita be sp-S01?

the macro looks good, but…

I’d say depth of field …
By: R. E. Tulloss (ret)
2012-05-28 22:00:35 CDT (-0400)

is a bit of a problem sometimes. Some cameras are inflexible on this issue with flash. Closing down the lens opening (which will require slower shutter speed) really can do a good job on improving depth of field. Using natural light in open shade with some reflectors to eliminate shadow and brighten the subject can improve things a great deal. Your efforts to document the mushroom from many angles is very admirable.

R

Nice pictures of the bulb [edited spelling]
By: R. E. Tulloss (ret)
2012-05-28 21:50:54 CDT (-0400)

The photographs of the bulb that were added show good detail. I think Amanita sp-S01 would be a good first choice in this case. Despite the fact that most of the spores illustrated are not in lateral view (standard for measurement), I still get the impression that some of the spores have length about 1.5 times width; and that would be very much on target for the length/width ratio (Q) of sp-S01.

Very best,

R.

Macro pictures of bulb
By: Christine Braaten (wintersbefore)
2012-05-28 18:18:18 CDT (-0400)

added.

I thought…
By: AmatoxinApocalypse (AmatoxinApocalypse)
2012-05-28 15:02:54 CDT (-0400)

I thought this was a good candidate for Amanita sp-S1., I have been finding something very similar to these down here, lots of them out at the moment. But I guess the spores are wrong for Amanita sp-S1

http://www.amanitaceae.org/?Amanita+sp-S01

Also where is the cup like volva which section vaginatae is known for, its hard to see what’s going on with the base in that first pic. Might I add, if you take a picture of the base of an Amanita try taking it at an angle to show more features. If you take the pic head on it will not show rings or other important identifying features because sometimes the flash will blend the whites together.

Created: 2012-05-28 11:53:44 CDT (-0400)
Last modified: 2012-05-30 08:20:43 CDT (-0400)
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