Notes:
> A single fruiting body growing at the base of a small stump surrounded by pitch pines in the entrance area of an FPP section dubbed “The North Gate”.
> Cap diameter = ~7 cm; stipe (apex to upper bulb) = 11.5 cm long
> The bulb = ~2 × 2 cm.
> A piece of grayish universal veil was observed on the cap surface.
> About 1/3 of the total stipe length was subterranean.
> Realizing a certain degree of morphological semblance between this mushroom and that of obs 175241 & obs 174500, the bulb was carefully excavated and examined for the presence of the yellowish universal veil; none was found.

Microscopy:
Amyloid in Melzer’s;
[20/1/1]: L x W = (7.0-) 7.4-8.6 (-9.1) x (4.9-) 5.1-6.3 μm;
L’ x W’ = 8.0 × 5.5 μm;
Q = 1.37-1.50 (-1.63) — a very tight group of Q values;
Q’ = 1.44
All measured spores are ellipsoid, with exception of the longest spore (9.1 μm) that is marginally elongate.

Images

Mounted in Melzer’s and viewed at x1000; 1 div = 0.465 μm
Mounted in Melzer’s and viewed at x1000; 1 div = 0.465 μm

Proposed Names

91% (2)
Recognized by sight
Used references: obs 174500
Based on microscopic features: Spore measurements
Based on chemical features: nrITS & nrLSU sequences — see comments below

Please login to propose your own names and vote on existing names.

= Observer’s choice
= Current consensus

Comments

Add Comment
Thank you, Rod,
By: I. G. Safonov (IGSafonov)
2017-12-18 20:35:57 CST (-0500)

for the good news.
I think the gestalt of this critter has taken a more defined shape now when we have at least two confirmed collections accompanied by photos. As before, you are welcome to use any of these images for your website.
I agree that single nucleotide polymorphism events (if I use this term correctly in this case) in conserved areas of the ribosomal genes are a cool ways of supporting species or perhaps even series of species within a genus. I’ve seen these SNPs in the primer regions of LSU sequences (e.g., ITS4B & ITS6R) of some boletes with some regularity, and I feel there are certain patterns there, too, if one takes a closer look. Of course, it’s also possible that at least some of them are just random.

As Igor predicted earlier today, sequences from the new DNA shipment of…
By: R. E. Tulloss (ret)
2017-12-18 18:39:36 CST (-0500)

recent days has produced evidence that this collection is Amanita umbilicata. The nrITS sequence is 99.8% identical to the previous sequence for the species that we had prior to the most recent data shipment. The nrLSU sequence contains a rare variant of the left hand terminal string. Which is a cool way to support ID of this species. It is TTGGCCTCAAATCA instead of TTGACCTCAAATCA (one character difference, but striking). The nrLSU sequence is a 100% match to the sequence reported earlier from the same batch of data (obs 174500).

A good day opening molecular Christmas and Hanukkah presents.

Very best,

Rod

Thanks Igor,
By: groundhog
2014-10-14 12:24:39 CDT (-0400)

This material has been received and accessioned to Rod’s herbarium. We have scheduled it for DNA sequencing.
-Naomi

Spore measurements / pix posted
By: I. G. Safonov (IGSafonov)
2014-09-13 00:31:49 CDT (-0400)