Notes:
Growing near Red Oak, Red Maple, American Beech primarily. About ten inches in height. Two inches? diameter on large cap.

Images

Proposed Names

ret
12% (2)
Based on chemical features: Molecular studies
ret
91% (2)
Based on chemical features: distinct by a combination of morphology and genetic sequences see particularlarly the picture of the cap in the second photo.

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= Observer’s choice
= Current consensus

Comments

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Another gray one here:
By: Garrett Taylor (cappy)
2020-02-13 22:36:59 MST (-0700)
GB sequences
By: I. G. Safonov (IGSafonov)
2019-12-18 19:29:23 MST (-0700)

The two accessions are still labeled as ‘sp-N66’. It wold be nice to have the titles corrected to ‘sp-N68’ to avoid confusion between the two taxa when doing BLASTn.

Amanita “__sp-N68_” must be permanently separated from sp-N66.
By: R. E. Tulloss (ret)
2019-09-17 14:22:16 MDT (-0600)

The color and striae of the caps as well as the strong difference in the ITS2 region of the proposed fungal barcode gene provide two strong reasons. This is the case even if the rLSU sequences of the two species differ only slightly.

Sorry for the confusion. A new set of data nailed the genetic part of the argument.

Very best,

Rod

The good color shots in the field will enrich the illustrations for “sp-N66.”
By: R. E. Tulloss (ret)
2018-12-14 09:49:12 MST (-0700)

R

sp-N68” must be merged back into “sp-N66”
By: R. E. Tulloss (ret)
2018-12-14 09:43:02 MST (-0700)

A review of new data and a check of my sequence editing by Dr. Kudzma. Says I was on target the first time round. So I eat a little crow. Corrections will be made on GenBank as necessary.

Very best,

Rod

We created a new temporary code for this material.
By: R. E. Tulloss (ret)
2018-09-02 13:24:01 MDT (-0600)

R

After further study, I withdraw my suggestion that this is “sp-N66”.
By: R. E. Tulloss (ret)
2018-09-02 13:09:37 MDT (-0600)

The cap color is wrong, the striations are too long in proportion to the cap radius.

I’m sorry for creating a misdirection.

Very best,

Rod

A recent shipment of new DNA data helped to resolve a possible placement for…
By: R. E. Tulloss (ret)
2018-08-21 08:20:00 MDT (-0600)

the voucher of this collection:

http://www.amanitaceae.org?Amanita%20sp-N66.

It appears that this species has now been collected before in Connecticut and New Jersey as well as in New York. It took a little while to work this out because (going over the details of original date) there are two character positions in the nrLSU sequence that seem to be variable within the species.

The present species has a grayer cap than in the other two collections.

Very best,

Rod

Another analysis not using BLAST comparison,…
By: R. E. Tulloss (ret)
2017-04-18 18:24:17 MDT (-0600)

the closest taxon was estimated to be Amanita sp-N66.

R

The data does not include the Penetratrices variation in the…
By: R. E. Tulloss (ret)
2017-04-18 17:55:42 MDT (-0600)

“beginning” (5’ end) of the nrLSU sequence.

R

Thanks again, Garrett.
By: R. E. Tulloss (ret)
2017-04-18 17:53:45 MDT (-0600)

R

We have received both nrITS and nrLSU sequences from this material. <<EDITED
By: R. E. Tulloss (ret)
2017-04-18 17:53:20 MDT (-0600)

The sequences are similar to some others that we have derived in the past, but no clear picture of relationships appears after working on the data for awhile. I think this one may be one for which we have to put the data into our data base and wait for a second example to come along. Our herbarium accession number is RET 697-2. This is the way we will identify the collection for the time being.

With regard to the LSU sequence the closest “matches” include taxa from the northern tier of U.S. states: sp-WA03, populilphila, and sp-MN03. The genetic distances to these taxa from the present material ranges form 0.8% to 1.0% or a little greater.

We don’t know about variability of rDNA in the present species. It shows similarity to one sequence of populiphila, which has a moderate degree of variability in its nrITS (compare all the sequences for the species we’ve submitted to GenBank). On the other hand it also shows the same degree of similarity to one of our unnamed singletons (with herbarium accession number RET 506-1), which is a mature specimen of a white to creamy species of sect. Vaginatae with a weak or crumbling volva (collected in NJ).

That’s what we can say for now.

Very best,

Rod

Thanks Garrett,
By: groundhog
2015-07-28 09:30:05 MDT (-0600)

We have received this material and it has been accessioned to Rod’s herbarium. We have scheduled it for DNA sequencing.
-Naomi

Thank you Garrett.
By: R. E. Tulloss (ret)
2015-06-24 16:13:15 MDT (-0600)

Your material was received today.

Rod

I certainly would be interested.
By: R. E. Tulloss (ret)
2014-09-05 11:28:13 MDT (-0600)

We are building up a library of DNA on, among other things, white-capped species of the Vaginatae.

Your material will make a very interesting addition to our data.

I suspect that your cap width is based on measuring across the bottom of the cap when it was “bell-shaped.” Send me an email via MO so that I can see your email address and send you a sheet of paper rulers (cm scale) that you can use to measure along the surface of the cap even when the cap has not expanded fully. When you do this, also measure the lengths of the striations on the cap edge (a range of lengths may be seen for the striations). This will allow us to compute a range for the ratio of striation length to cap radius…a useful macroscopic character.

Very best,

Rod

Red tint: I think it was an artifact
By: Garrett Taylor (cappy)
2014-09-04 21:33:39 MDT (-0600)

I will grab this one tomorrow. I will send it to you if you are interested, Rod.

Is the pinkish tint on the stem something that you saw with the naked eye or is it an
By: R. E. Tulloss (ret)
2014-09-04 20:44:16 MDT (-0600)

artifact of the camera. Since it seems to move about from picture to picture, I think it is probably in the camera.

This is very interesting looking.

The posting shows there is no herbarium specimen. Is that correct?

Very best,

Rod Tulloss

Created: 2014-09-04 15:53:29 MDT (-0600)
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