Collection location: Michigan City, Indiana, USA [Click for map]
The Top BLAST match was Amanita cf. virosa Montoya (sequence size was 245, 84% max identity):
Habitat- sandy soil, oak and pine woods
Spore print- whitish cream
Spores- 11.62-15.77×4.15-4.98 microns
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If I remember correctly, Cristina started it as a spreadsheet after a discussion between us about getting more out of the nrITS data. As you probably can tell, the last update of the page was by Naomi, who went back to the spreadsheet mode and then updated the page. The page is now, again, out of date because it doesn’t show the correct plenitude of Vaginatae with the 28S 5’ motif TCTGACCTCAAATCA. That is a fascinating group with very broad distribution in the world. A lot of species in the group came to my attention through MO. Another contribution of the MO collectors to the Amanita data mavens.
That is a pretty stellar page.
related to nrITS in Amanita:
so I zapped it to avoid confursion.
I agree with Steve. The sequence looks like an initial segment of nrITS, but there is a minor problem and more significant problems that can be seen even without an alignment.
There is a small flaw in the fragment of 18S that is often the result of using only a forward read to try for an nrITS sequence. The 5’ (right hand end) motif of 18S should terminate with …GGAAGGATCATTA. What you have is missing one of the first pair of A’s in the motif. This is often missed by a forward read because it is too near the beginning of the read. A reverse read will usually pick it up correctly if the sequence is relatively clean.
Following GGATCATTA, you have “TTAG” It is very unusual for the beginning of ITS1 not to have a “G” in the third position. So without alignment there may be something fishy here.
Also, the beginning of ITS1 is often related to the section of the genus that is represented by your sample. In section Phalloideae, the most common left hand terminal characters are “ATG…” and “CTG….” We know of only one with “TTG” (and this is east Asian material). So, again, just by examination of the sequence, it would be a good idea to have a “do over.”
and am going to run it again.
In the technical descriptions on www.amanitaceae.org, the order of description of the elements of the stipe is (1) stipe description above the bulb excluding veils, (2) bulb excluding universal veil, (3) context, (4) partial veil, (5) universal veil.
This is the standard approach that I’ve developed and used over the last 36 (roughly) years. It’s a divide and conquer approach, set up so that comparisons between species are easier for a reader to make.
Except for item (1), the descriptions of parts begin with the name of the relevant part in italic type. The idea is to help the reader find the start of the description of a specific part in a long overall description of the stipe.
The data you were looking for is here:
…; universal veil as membranous to submembranous, limbate volva, torn into irregular lobes, smooth surfaced or impregnated with sand, white with occasional reddish or reddish brown stains, sometimes a pinkish tint on the interior surface, rarely(?) “bruising pinkish” (Murrill, regarding F17658), thin (less than 1 mm thick), upstanding at first and separated from the stipe barely (often with no evidence of limbus internus) or (less often) by width of complete, or rounded remnant of, limbus internus, adnate for about half to two-thirds of limb height, not clearly having an internal layer; height from highest tip of limb to bottom of bulb 33 – 62 mm (Beardslee: free limb 20 – 40 mm long).
The upshot is that there is a very pronounced, upstanding volval limb present in cylindrispora.
Unfortunately, your problem is not yet resolved.
I keyed Amanita cylindrispora backwards on mushroomexpert.com and found I that it did not match mine because in the key leading up to A. cylindrispora I would have to choose “Stem base without a sack-like volva” and mine does have a sac-like volva. The description on http://www.amanitaceae.org/?Amanita%20cylindrispora does not say that directly it says “bulb 23 – 34 × 13 – 23 mm (Beardslee: bulb 50 – 70 mm long), rounded below (subclavate) to napiform to very slenderly fusiform to subfusiform, subradicating to radicating, occasionally doglegged, with white mycelium at base”. I guess you really have to know your terminology for this one! But hey, I learned something new!
I’ll try contacting you through MO’s email function. If you’d like to lay out what you know and what your problems are, I’m interested in helping you solve this one.
Yes, the soil is pretty sandy. It in my yard which is next to a wooded sand dune (mainly oak, pine and sassafras).
I’ll have to try the KOH next week but, would it still react the same on a dried specimen?
A.cylindrispora or A. virosiformis sound the most likely, but some things just don’t line up. I dont know but this one is sure giving me a lot of trouble!
It looks like the mushroom was in dark sand. I see that you’re not very distant from a lake shore area.
Did you try a drop of KOH on the cap?
Is that correct?
If so, try
in section Lepidella.
one of the limbate species in section Lepidella.
The extremely narrow spores are the clue.
For links to descriptions for the latter:
For the former, try
(and my apologies for the crumby picture…I’ve only found the species once).
Let me know how it goes.