Observation 192101: Clitocybe (Fr.) Staude

When: 2014-10-03

Collection location: Deep River County Park, Hobart, Indiana, USA [Click for map]

Who: Marielle Biscocho (mbiscoch)

No specimen available

-This specimen was associated with the soil, and it was growing in solitary. —When I smelled it, the scent reminded me of freshly picked corn.
-The diameter of the pileus was about 2.9 cm.
-The cap was mostly light tan with some brown in some areas.
-The cap was smooth and greasy.
-The pileal shape was broadly convex, and the margin was also smooth.
-The flesh was observed to be a yellow beige.
-There weren’t any notable changes when the specimen was damaged.
The light brown stipe was a little bit off-center, but the stipe was closer to the center rather than the lateral areas.
-The stipe was measured to be about 3.0 cm in length and 0.6 cm in width.
-When the stipe was halved, it was observed to be hollow.
-The base was inserted.
-The surface of the stipe felt somewhat greasy, and it looked fibrillose.
-The light tan/beige gills were adnexed and even. There were four lamellae tiers arranged in a regular pattern.
-Many basidia were present when the specimen was observed under 40x magnification.

Species Lists


Mb66 basidia no spores.jpg
Basidium filled with spores (40x magnification)

Proposed Names

6% (3)
Recognized by sight
43% (3)
Used references: My DNA sequencing and blast scores
-35% (2)
Recognized by sight

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= Observer’s choice
= Current consensus


Add Comment
The peaks for the DNA sequencing were pretty prominent for the most part.
By: Marielle Biscocho (mbiscoch)
2014-12-13 22:15:34 MST (-0700)

From the very beginning, I made sure the labels were correct from the extraction process to PCR to DNA sequencing. Unless there was contamination, there shouldn’t have been a case where something was inaccurately placed. The sequencing wasn’t perfect, but the identified peaks were easily picked up.

Is it possible that you mixed up the photos and sequenced sample?
By: Jacob Kalichman (Pulk)
2014-12-13 21:39:43 MST (-0700)
DNA Sequencing and Blast score
By: Marielle Biscocho (mbiscoch)
2014-12-12 18:11:46 MST (-0700)

My sequence consisted of 358 bp’s.

When I researched the GenBank for my sequence, the top 10 descriptions were mostly of Clitocybe species.
BLAST max score:593
BLAST total score: 593
BLAST query coverage: 91%
BLAST max identity:99%

Could you provide your sequence(s) or any other details?
By: Jacob Kalichman (Pulk)
2014-12-12 15:49:16 MST (-0700)
Couple notes
By: Jacob Kalichman (Pulk)
2014-12-06 19:18:46 MST (-0700)

- All your detailed notes are awesome!
- Call unidentified mushrooms with gills Agaricales sensu lato (as Could Be, so the observation will get titled with more specific suggestions)
- I think those are 400x magnification, 40x objective lens * 10x eyepiece
- The plural of “basidium” is “basidia”