Notes:
Growing on the ground with Live oak being the most dominant tree.
Total height about 10 cm.
Spore print white and spores not amyloid.
Spores ~ 9.0-11.0 X 8.1-10.0 microns, globose to subglobose and smooth.
Q(ave) = 1.07.
Only a single fruit-body but thought the light color and thick volval patch were interesting and rather unique for the Grisettes in this area.

Images

Spores in Congo red + KOH @ 1000X.

Proposed Names

60% (5)
Recognized by sight
Used references: Comments by RET
-19% (3)
Recognized by sight: What’s the difference?
ret
19% (4)
Based on chemical features: This species does not seem to match anything described in the literature and its nrITS and nrLSU sequences do not match sequences posted in GenBank or unposted sequences in RET’s local database. Hence a new cryptonom. temp. is proposesd.

Please login to propose your own names and vote on existing names.

= Observer’s choice
= Current consensus

Comments

Add Comment
THanks Rod
By: Ron Pastorino (Ronpast)
2019-05-23 18:41:06 CDT (-0400)
Another recently sequenced collection (MO 306162) clearly demonstrates that…
By: R. E. Tulloss (ret)
2019-05-23 11:14:05 CDT (-0400)

Amanita crassiputamen and Amanitasp-C23” are one and the same species. The genetic evidence is now quite convincing.

Ron, I apologize for not recognizing your correct identification of the voucher for this observation. You were right.

Thank you very much for sharing so much of your material with me.

Very best,

Rod

As always, Ron,…
By: R. E. Tulloss (ret)
2018-11-10 22:11:51 CST (-0500)

I very much appreciate the challenges you send me.

Very best,

Rod

Thanks Rod,
By: Ron Pastorino (Ronpast)
2018-11-09 15:02:02 CST (-0500)

Will keep a lookout for more of this creature.

nrITS and nrLSU have received GB accession numbers and are posted here.
By: R. E. Tulloss (ret)
2018-11-09 14:36:01 CST (-0500)

Very best,

Rod

I decided to propose “sp-C23” as a temporary code for this critter.
By: R. E. Tulloss (ret)
2018-10-25 10:39:00 CDT (-0400)

R

Well, some more time has passed. More sequences are in our local database…
By: R. E. Tulloss (ret)
2018-10-25 10:25:28 CDT (-0400)

and more sequences are in GenBank. It looks like crassiputamen no longer is the best match for either the nrITS or nrLSU from the present species.

It would appear that there is no really close match for the sequences we have from the voucher for this observation.

I am going to go over the data again. I don’t think this species fits anything in the literature. It sequence data is not a match to any sequence data that has been posted or that I have seen and not yet posted.

More later.

Very best,

Rod

And A. calyptratoides is a species in section Caesareae with a stem…
By: R. E. Tulloss (ret)
2017-07-05 00:18:50 CDT (-0400)

developing the appearance of a wax candle into which the partial veil appears to melt. A very distinctive species in the western U.S. The only similar species that I know of is a close relative known from the central volcanic zone in Mexico—-with the provisional name “tlaxcandela.” It is very similar to calyptratoides to the naked eyes; however, the two species were distinguished on molecular grounds.

Very best,

Rod

Microscopically there is a large difference
By: Ron Pastorino (Ronpast)
2017-07-04 19:06:11 CDT (-0400)

in spore shape. A crassiputamen spores are globose to subglobose while A calyptratoides are ellipsoid to elongate.

Yes…Quercus agrifolia
By: Ron Pastorino (Ronpast)
2017-07-04 18:09:23 CDT (-0400)

would be my best guess. The area is predominately live oak/madrone habitat.
Thanks Rod.

Is the associated live oak…
By: R. E. Tulloss (ret)
2017-07-04 16:58:31 CDT (-0400)

Quercus agrifolia?

Very best,

Rod

I added this collection to the crassiputamen web page. <<<EDITED
By: R. E. Tulloss (ret)
2017-07-04 16:54:31 CDT (-0400)

I checked the spores (I measured 20 from the spore deposit) and found that they were immature, but had the normal range of Q for crassiputamen. The universal veil was felted/submembranous and included some thick-walled cells as slightly inflated hyphal terminal or subterminal cells and smallish globose to pyriform inflated cells. These match the cells reported from the volva of the collection that will be the holotype of crassiputamen.

I conclude that at least sometimes the early stage of the cap is grayish pale tan, while the mature cap is somewhat more darkly pigmented (brownish gray, causing it to be mistaken at least once for A. constricta by the authors of the latter species. The current known range of the species extends from Marin Co. to San Mateo Co.

Very best,

Rod

That’s a good way of making a judgment call.
By: R. E. Tulloss (ret)
2017-06-16 21:23:43 CDT (-0400)

Good idea, Ron.

Very best,

R

Thanks Rod,
By: Ron Pastorino (Ronpast)
2017-06-16 18:16:50 CDT (-0400)

I’ll go with A. crassiputamen as “Promising” for now. Many “Promising” id’s on MO with a lot less back up information.

Sorry that we didn’t report the date of accessioning….EDITED
By: R. E. Tulloss (ret)
2017-06-15 12:39:26 CDT (-0400)

We don’t record that date on herbarium labels; therefore, I can’t correct the record on the observation.

From the container in the herbarium, I can tell you with high probability that the collection was accessioned in late December 2014 or early January 2015.

Very best,

Rod

This material was accessioned & we have both nrITS & nrLSU sequences…EDITED
By: R. E. Tulloss (ret)
2017-06-15 12:30:29 CDT (-0400)

…from it.

The nrITS is very similar to the sequence we have from the 1989 collection of A. crassiputamen.

By “very similar” I mean the pairwise identity is 100%. There are two positions in the sequence where one of the pair is ambiguous (say, C-or-T) and the other is not (say, C). This is considered pairwise identity since in a case of heterogeny a nucleotide position may be read (same example) as C or as T or as C-or-T.

Unfortunately we have no nrLSU from the older specimen. We will look into getting one with Linas’ current techniques.

Since we have a full technical description of that material, I will check at least a few of the key anatomical characters against your material. The cap of your material is paler that the grayish brown cap reported for RET 092-1 (1989 collection). This could be due to natural variation…but, of course, we only have two examples in hand.

If I have any progress on this matter, I will advise you further.

Very best,

Rod

Your material has arrived in Roosevelt.
By: R. E. Tulloss (ret)
2014-12-15 17:05:46 CST (-0500)

Thank you, Ron.

The material will be accessioned in the near future.

Very best,

Rod

That’s great, Ron.
By: R. E. Tulloss (ret)
2014-12-11 07:26:03 CST (-0500)

I look forward to seeing the material.

Very best,

Rod

Sure Rod,
By: Ron Pastorino (Ronpast)
2014-12-10 22:15:20 CST (-0500)

I’ll sent the whole thing(two-halves).

This looks very interesting.
By: R. E. Tulloss (ret)
2014-12-10 21:52:23 CST (-0500)

Would you be will to share a bit of this one?

Very best,

Rod

Created: 2014-12-10 20:32:50 CST (-0500)
Last modified: 2019-12-18 21:06:25 CST (-0500)
Viewed: 308 times, last viewed: 2020-01-21 21:57:16 CST (-0500)
Show Log