These, and many others were growing around a Live oak.
Conditions were very wet, so I picked 2 groups of three as shown in the photos, that were relatively in good shape. These were less gray than many of the others, which looked more like typical Grisettes and which we have been calling A. vaginata or A. constricta.
Spores were ~ 9.0-12.0 X 9.0-11.8 microns.


Spores in Congo red + KOH @ 1000X.
Spores in Congo red + KOH @ 1000X.

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Thanks Rod,
By: Ron Pastorino (Ronpast)
2019-12-18 07:41:21 MST (-0700)

We might be getting to the point where we can almost ID this species in the field with some degree of confidence.

The images are lovely, Ron.
By: R. E. Tulloss (ret)
2019-12-17 16:10:56 MST (-0700)

Thanks again for the wonderful material.

Very best,


We have derived a lengthy joined nrITS and nrLSU sequence for the voucher of this observation.
By: R. E. Tulloss (ret)
2019-12-17 16:03:00 MST (-0700)

It is confirmed as a specimen of Amanita crassiputamen.

Very best,

Rod Tulloss

Thanks Ron,
By: groundhog
2015-01-13 12:42:28 MST (-0700)

We have received this material and accessioned it to Rod’s herbarium, It has been scheduled for DNA sequencing.

You’re welcome, Ron.
By: R. E. Tulloss (ret)
2014-12-18 18:19:37 MST (-0700)

Oh, I think it’s more than a handful. Sometime I’ll have to count the number of provisional name Alexander Smith had from Washington, Oregon, and California. I think you need more than two hands’ worth of fingers to count them. I think I can count 13 on WAO right now.

It will take a while to sample multiple specimens from the many suspected species, the types of names, and the many other tasks that need to be done; so this is a longish term project even if I were only working on the Vaginatae.

This is the advantage of having a mutable on-line monograph-like site that you can constantly revise and expand. People can watch science happen. Today we just put up 30 new GenBank numbers. GenBank won’t post the sequences for weeks, but folks can see what’s coming.

Anybody wanting to see sequences for a favorite Amanita or Limacella and lacking a local connection (or wanting to see their data shared openly on WAO) can send us material to go into one of our queues.

Very best,


Thanks Rod, since we only have a handfull
By: Ron Pastorino (Ronpast)
2014-12-18 16:57:36 MST (-0700)

of Vaginatae in California, and the gray Grisettes can be ubiquitous, I tend to ignore them unless they look unique.
So maybe we have a little more than a handfull if we look closer?
It would be nice to see how different from each other these Grays actually are and were they stand in the overall picture.

The Vaginatae have been so ignored!
By: R. E. Tulloss (ret)
2014-12-18 10:11:38 MST (-0700)

Hello, Ron.

DNA is really offering a primary sorting tool that was lacking. Every time we get a shipment of sequences derived from samples taken from Vaginatae, it’s like opening presents. “Never seen this before, never seen this before, never seen this before,…HOLY MACKEREL this is that same weird thing that Anna sent us from South Dakota and this one’s from New Mexico!”

That little bit of (barely) imagined dialog is the synopsis of a true story. Thanks to Anna Gerenday, Robert Chapman, and MO we have this page:

(Look at the weird stipe covering and the ellipsoid spores.)

Thanks for your support, Ron. The images, the well-dried collections, and the notes make for a very nice collection of very usable data time after time.

Very best,


Rod, yes, I’ll be sending off the lot
By: Ron Pastorino (Ronpast)
2014-12-18 08:45:26 MST (-0700)

to you shortly.
With the recent rains, there are plenty of these vaginata types popping up.

I see that the spores are distinctly non-globose on average.
By: R. E. Tulloss (ret)
2014-12-18 07:56:28 MST (-0700)

This should add to the ability to distinguish the species within sect. Vaginatae.

Very best,


Very interesting, Ron.
By: R. E. Tulloss (ret)
2014-12-17 22:41:23 MST (-0700)

I am interested to see if the slightly different cap colors matter taxonomically. If you have the opportunity, could you spare some material from each of the two sets of three for my study?

It looks like some really robust material is appearing this year.

Very best,


Created: 2014-12-17 19:45:36 MST (-0700)
Last modified: 2019-12-18 07:41:21 MST (-0700)
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