When: 2015-07-02
Collection location: Franklin Parker Preserve, Speedwell, New Jersey, USA [Click for map]
Who: I. G. Safonov (IGSafonov)
Project: Northeast Bolete Consortium
Notes:
> A single large and robust basidiome growing on the edge of a trail in mixed pitch pine & oak woods in the Speedwell section of FPP.
> I’ve posted this species several times on MO — all collections are from the NJ Pine Barrens.
> This is the earliest fruiting I have ever observed.
> The cap of this young specimen was almost 5" in diameter; the stipe was ~7" long and 1.5" wide.
DNA Sequencing Discussion (final update 6-Nov-17):
> A clean and contiguous 1368 bps nrLSU sequence has been obtained for this material. The first 70+ bps from the beginning of the LSU region are missing.
There is a single ambiguous character, a “Y”, corresponding to either of two the pyrimidine bases, cytosine “C” or thymine “T”.
> A GenBank BLAST search of the full-length sequence returned no meaningful hits.
> A GenBank BLAST search of a bps sequence fragment (the first 886 bases) returned a nearly perfect match (867/868 bps overlay, 99.9% identity match) to Boletus oliveisporus voucher DPL6823 [accession #KF030254]. The next several hits with identities in the 97-98% range are members of Neoboletus and Rubroboletus.
> Morphologically and genetically, B. oliveisporus is likely a member of the unresolved “Pulveroboletus Group”, a dustbin containing many generic clades that haven’t been assigned to any of the 7 existing subfamilies within the Boletaceae.1 Its gestalt morphology suggests it belongs in a yet to be named generic clade.
Case closed.
1 “Molecular Phylogenetic Analyses Redefine Seven Major Clades and Reveal 22 New Generic Clades in the Fungal Family Boletaceae”, Fungal Diversity 2014, 69(1), pp. 93-115.
Species Lists
Images
| User’s votes are weighted by their contribution to the site (log10 contribution). In addition, the user who created the observation gets an extra vote. | |||||||||
| Vote | Score | Weight | Users | ||||||
|---|---|---|---|---|---|---|---|---|---|
| I’d Call It That | 3.0 | 8.56 | 2 | (MsMucor,IGSafonov) | |||||
| Promising | 2.0 | 0.00 | 0 | ||||||
| Could Be | 1.0 | 0.00 | 0 | ||||||
| Doubtful | -1.0 | 0.00 | 0 | ||||||
| Not Likely | -2.0 | 0.00 | 0 | ||||||
| As If! | -3.0 | 0.00 | 0 | ||||||
| Overall Score sum(score * weight) / (total weight + 1) |
2.69 | 89.54% | |||||||
Comments
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According to Wu et al. (2014), Cyanoboletus (clade 48) and Lanmaoa (clade 49) are very closely related.
that is clean and dry. obs 170957
collections of pulverulentus.
all components of this mushroom bruise deep blue, including the cap cuticle; the pores are exceptionally sensitive — you can drag a hair across the bright yellow surface and it will blue. The flesh immediately stains blue first and then transitions into brown after a few minutes. Kuo says C. pulverulentus is similar, but stains green-blue, and is much smaller in size.
It’s on my wish list for sequencing.
bruise like crazy? Lots of black, and other shades, all parts? Wondering how close it is to pulverulentus.
This mushroom is an amazing blue stainer, especially on the pore surface. According to a Texas field guide, it’s edible. I haven’t tried it, thought it’s tempting. The flesh is dense and a single large mushroom can easily make a meal. :-)
I hope to see this one someday
LSU sequencing confirms that this is indeed B. oliveisporus.