Collection location: Meadowood Park, Mendham, New Jersey, USA [Click for map]
Project: Northeast Bolete Consortium
Collected at the NJMA foray by unknown participant(s).
DNA Sequencing Results & Discussion (last update on 17-Jan-18):
> A clean and contiguous nrLSU sequence consisting of the first 1445 bases was obtained for this material. There is a single ambiguous character, a “Y”.
> As expected, the full length sequence did’t return any meaningful hits from a BLAST nucleotide search in GenBank (bolete sequences posted there are much shorter).
> A BLAST search of a sequence fragment (first 974 bases) yielded the following 3 top hits:
Boletus subvelutipes #AY612804 (USA) = 99.9% similar (1 base off)
Boletus vermiculosus #DQ534646 (USA)= 99.1% similar (8 bases off)
Sutorius tomentulosus #KF112323 (China) = 98.9% similar (9 bases of)
> A sequence fragment consisting of the first 980 bps is a 99.9% match to the LSU sequences of Eva Skific’s obs 279688 and obs 281329 from SE Canada.
> : In my understanding, the mushroom in this observation doesn’t really fit the morphological species concept of B. subvelutipes, so I am willing to bet that AY612804 is likely to be a misidentification. The LSU data suggest that, as it’s likely the case with other red-pored boletes from NA, the vermiculosus/vermiculosoides series also falls into the Neoboletus-Sutorius clade. This collection is likely to be contaxic with obs 279688 and obs 281329.
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I am just a messenger — all the kudos should go to Dr. K who made all of this possible…
Martin, I agree. However, I don’t know how to make this info more visible to folks from the academia. The vouchers, raw data and annotated sequences for all the shooms I post on MO are available for anyone who is interested.
It’s great to see this type of work on MO. We should figure out a way to share this observation with the folks you submitted the samples you compared with on GenBank. They need to document their morphology in this way.
I am glad you are getting good results.