Notes:
A single immature fruiting body growing in grass under an old oak and other hardwoods.

DNA Sequencing Results & Discussion:
> A clean, contiguous nrLSU sequence of the first 1448 bases was obtained from this material. There are no ambiguous characters; it’s a single haplotype.
> Curiously, unlike my previous experience, a GenBank BLASTn search of the full-length sequence gave a top scoring hit that made some sense — Boletus hiratsukae. This bolete is endemic to Japan and is a bona fide member of the “Variipes-group” – one of three clades in the porcini sensu stricto lineage of Boletus sect. Boletus defined in the studies by Dentinger et al. (2010) and Yang et al. (2012) – and the closest genetic relative of North American B. variipes.1,2 Its sequence of 1244 bps was long enough to show up in the search.
> A GenBank BLASTn search of the first 966 bases returned two very close matches:
1) #JQ327014: B. variipes var. fagicola voucher 4249 = holotype collected by A. H. Smith in 1968 in MI (878/880 = 99.8% similarity)
2) #HQ161846: B. variipes voucher BD378 collected/identified by B.T.M. Dentinger in PA (872/875 = 99.7% similarity)
> Conclusion: Even without the DNA evidence, placement of this bolete into the “B. variipes group” based on morphology made a lot of sense.3,4 The molecular evidence based on a single locus sequence gives a slight preference to var. fagicola, though Peck’s parent taxon and Smith & Thiers’ var. fagicola are likely to be genetically conspecific.

References:
1 B.T.M. Dentinger et al.: “Molecular Phylogenetics of Porcini Mushrooms (Boletus section Boletus); Mol. Phylogenet. Evol. 2010, 57, pp.1276-1292.
2 Z.L. Yang et al.: “DNA Sequence Analyses Reveal Abundant Diversity, Endemism and Evidence for Asian Origin of the Porcini Mushrooms”; PLoS One 2012, 7 (5), e37567.
3 http://mushroomobserver.org/name/show_name/62292
4 Obs 282673 and discussion therein

Species Lists

Images

Natural light/no flash
Natural light/no flash
Natural light/no flash
Copyright © 2015 Prof. Bryn T. M. Dentinger

Proposed Names

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= Observer’s choice
= Current consensus

Comments

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Specimen accessioned to the University of Utah fungarium
By: Dan Molter (shroomydan)
2018-10-11 04:17:46 JST (+0900)

This specimen has been accessioned to the UT fungarium with the following number:

UT-M0000520

Half of this collection…
By: I. G. Safonov (IGSafonov)
2018-04-12 01:43:34 JST (+0900)

…was transferred to Dr. B.T.M. Dentinger at UMNH in March 2018. The other half is still with the NJMA Herbarium.

Nice to see
By: walt sturgeon (Mycowalt)
2016-02-04 14:18:32 JST (+0900)

DNA almost confirms a macro identification, I think!

very good work
By: John Plischke (John Plischke)
2016-02-04 14:13:56 JST (+0900)

very good information to know. Glad to hear you are getting good results.

DNA discussion posted
By: I. G. Safonov (IGSafonov)
2016-02-04 13:13:25 JST (+0900)
Walter,
By: I. G. Safonov (IGSafonov)
2016-01-02 16:09:51 JST (+0900)

Your proposal did cross my mind when I was posting this observation; however, I didn’t think this specimen was sufficiently dark enough to pass as var. fagicola.
I submitted this one for sequencing (the LSU locus) a month ago. I don’t know if one non-protein coding gene is enough to separate variipes from variipes var. fagicola at the species level. I suspect it’s not, but I should get more info from Roy Halling to be certain.
In the humongous bolete LSU tree published in the Nuhn et al. 2013 paper, the two species form a distinct and well-supported clade within the Boletus sensu stricto group, meaning that they are “siblings”. The same paper lists the holotype voucher of var. fagicola collected by A. H. Smith in 1968 for which LSU, Tef1 and RPB1 sequences exist.