When: 2016-07-07

Collection location: Maple Shade Twp., Burlington Co., New Jersey, USA [Click for map]

(coordinates hidden from public)

Who: I. G. Safonov (IGSafonov)

Specimen available

Notes:
Same location and species as obs 207988. Phenolic odor. KOH is deep yellow on cap surface.

Proposed Names

20% (2)
Used references: MushroomExpert.com
Based on chemical features: KOH yellow on cap
63% (3)
Recognized by sight: Pigmented cap surface, bulbous stipe, fits Pennsylvania collections studied by RWK
Used references: KJ609480, 657/658, no gaps, 1 amb. base, A. cf. approximans, RWK_2064, Pennsylvania, RWK

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= Observer’s choice
= Current consensus

Comments

Add Comment
This sequence is included in a phylogenetic tree here:
By: Terri Clements/Donna Fulton (pinonbistro)
2020-10-30 03:20:36 IST (+0530)
Discussion of Sequencing Results
By: I. G. Safonov (IGSafonov)
2017-04-30 08:56:00 IST (+0530)

> A partial nrITS sequence has been obtained from this collection. It consists of the last 646 characters of this locus. Unfortunately, the extra long ITS1 region becomes nonspecific in the forward 5’—>3’ read, a feature apparently common in some species in sect. Xanthodermatei. The reverse read gave complete ITS2 and 5.8S regions, and the last 283 characters of ITS1. There is a single ambiguous base (an ‘R’) in the ITS2 region.
> A GenBank BLASTn search gave Agaricus cf. approximans as the top hit (several sequences from reliable vouchers from Drs. Kerrigan and Ortiz-Santana) with a 99.9-100% similarity range. This unpublished species is in Dr. Kerrigan’s Agaricus of North America book, p.146.
> “Your sequence is a perfect match for other cf. approximans collections, whereas at least two characters don’t agree with other relatives. The photos are also a match. The original A. approximans came from NJ.” — Dr. R.W. Kerrigan (personal communication)