Notes:
Pores reddish orange staining greenish blue when cut. Context is negative with application of FeSO4. Growing on the ground in oak woods.

Species Lists

Images

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Proposed Names

14% (2)
Used references: North American Boletes
Based on chemical features
30% (2)
Recognized by sight

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= Observer’s choice
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Comments

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DNA sequencing results and discussion
By: I. G. Safonov (IGSafonov)
2017-02-23 06:38:11 IST (+0530)

> A clean, contiguous, 1311-bps nrLSU sequence was obtained from this material. The first 130+ nucleotides (5’ end) are missing, but ~half of these are primers anyway. Alignment of this sequence with that of Boletus carminiporus/obs 244944 shows the overlapping regions to be 100% identical. For a discussion of GenBank BLAST search results, see the latter observation.
> A clean, contiguous, full-length 610-bps TEF-1-alpha sequence was obtained for this material. A BLAST search of the entire sequence revealed that the top 13 hits (overall score and identity) are all members of Lanmaoa; these include sequences of authentic vouchers of angustispora, asiatica, and flavorubra (all 3 are from Asia) as well as borealis, carminipes and pseudosensibilis from North America. The % similarity range for all these sequences is 95.6-98.3%. The top scoring hit (98.3% similar), however, was the North American L. roseocrispans (accession #KP327616). Incidentally, the same relationship was observed for nrLSU. This means L. roseocrispans, not the morphologically similar L. borealis, is the closest genetic relative of B. carminiporus.
The TEF-1-alpha locus is very conserved as it codes for a protein. While point mutations leading to non-crucial amino acid substitutions are possible (the protein is still functional), the greatest variety in the sequence comes from degeneracy of codons.
This sequence lends even more credence to the fact that B. carminiporus actually belongs in Lanmaoa.

Liz,
By: I. G. Safonov (IGSafonov)
2017-02-10 03:54:25 IST (+0530)

Looking at your photograph, the upper 10% of the stipe appears to be finely reticulated. The pic is a bit blurry, but it’s clear that the stipe is not smooth. Examination of other B. carminiporus observations (http://mushroomobserver.org/observer/observation_search?pattern=boletus+carminiporus) shows that reticulation is a variable morpho-trait in this bolete. In some cases, there is just a bit of reticulation at the apex or no reticulation at all. Some collections feature both smooth-stipe and reticulated-stipe fruiting bodies.
Lanmaoa borealis is an uncommon bolete with the distribution range restricted to the northern tier of the USA — Michigan and upper New England states. Ernst Both’s voucher, the only example that has been sequenced, comes from Erie Co., NY (near Buffalo). Since many collections of B. carminiporus have been recorded from MD, its occurrence in NJ is totally in the realm of possibilities.

Thanks Igor for sequencing this mushroom.
By: Liz Broderick (Liz Broderick)
2017-02-10 03:18:10 IST (+0530)

Thank you Igor for sequencing this colorful fungi-no arguing with DNA although no reticulations were observed on the stipe and it was found north of its usual range.

A nrLSU sequence…
By: I. G. Safonov (IGSafonov)
2017-02-10 01:42:37 IST (+0530)

derived from this material was 100% identical to Don Johnson’s obs 244944 (see the discussion therein). So, this is not L. borealis. Not surprisingly, a TEF-1-alpha sequence obtained from this collection (246870) didn’t match the TEF-1 trace of Ernst Both’s L. borealis voucher.
Both sequences and a more thorough commentary will be posted here later.

Tough call
By: I. G. Safonov (IGSafonov)
2016-08-09 09:37:47 IST (+0530)

No reticulation on the stipe and negative reaction to Fe(II) should rule out B. carminiporus. Liz didn’t see any reticulation, but the somewhat fuzzy picture of the stipe shows the surface somewhat “busy” in the upper half. I am not sure what to make of it. NAB says the range is NC down to FL, but northern distribution limits are unknown. With the climate change, it’s conceivable that southern species are creeping up north, especially along the coast, so B. carminiporus may already be here to join other “southerners”.
B. bicolor v. borealis is a northern taxon found in New England. On one hand, one can make the same climate change argument to erode at the southern range of distribution and shift it to the north. However, Liz has collected what appears to be B. bicolor v. borealis from this site in the past — see obs 174150.

The macrochemical tests support the bicolor var. borealis name.
By: Liz Broderick (Liz Broderick)
2016-08-09 08:56:05 IST (+0530)

I don’t think this is carminoporus because there is no reticulartions on the stem and the context of this specimen did not stain grey or bluish grey with FeSO4 as you would find with carminoporus.