using key E-2 ending up at sect. 25a in B-R-B on second thought it keys better with E-3 calling the pore surface orange.

I didn’t notice much of a change with KOH on the pileus, possibly just more red.
taste is mild and pleasant odor not unusual Stained blue fast.

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A new GenBank hit
By: I. G. Safonov (IGSafonov)
2017-09-24 19:32:29 PDT (-0700)

The posted nrLSU sequence is identical to accession #MF797695 (=Boletus sp. strain MGW1536 from Great Smokey Mountain National Park in TN) submitted to GB on 30-Aug-17 by Matheny, Trudell & Wood.

I’m catching up
By: Geoff Balme (geoff balme)
2017-05-10 04:58:56 PDT (-0700)

It takes time to agree.

I’m going to have to fire up some phylogenetics software soon – I’m very curious about limits to variation in these sequences.

By: I. G. Safonov (IGSafonov)
2017-05-09 14:18:05 PDT (-0700)

I already commented on the likely conspecificity of 247957 with 255442 (similar appearance and identical LSU traces). By the way, 249090 has unique LSU that doesn’t match anything I’ve sequenced so far.

I’m beginning to see a number of these
By: Geoff Balme (geoff balme)
2017-05-09 10:53:03 PDT (-0700)

bright blue stainers with yellow stems, red/orange pore surfaces, tannish-red top with the margin of sterile material . . . as being equivalent species.

obs 249090
obs 256101
obs 255247
obs 255442

Similar observation
By: Octavian Stan (octavstan)
2017-05-05 08:25:19 PDT (-0700)

Looks like one of my finds, obs. 223849

Thanks again for your work and
By: Geoff Balme (geoff balme)
2017-05-04 03:40:29 PDT (-0700)

thoughtful responses. I always knew it was difficult to know the mushrooms, but I had no idea how downright mystifying it is! :) even with collections and plenty of state-of-the-art evidence!

The blue-staining bright yellow stem with the red to ocher pore surface is going to be my guide. I may shoehorn a few, but in the field we’ll be close.

microscope purchase this year hopefully as well.

Not sure, Geoff, but subluridellus is probably the best fit IMO
By: I. G. Safonov (IGSafonov)
2017-05-03 20:54:26 PDT (-0700)

Morphologically it seems like a good match with the original concept of Smith & Thiers and the descriptions in NAB, BENA and Roody’s WV field guide. Mind you, M. Kuo’s page shows pix of boletes that are not consistent with that description.

I think that the defining feature of the bolete in this observation, as well as 255442, 248580 and other numerous collections posted by Dario Z., are the pale yellow to medium yellow stipe with no hint of red and the dark pink-red pore surface fading to pink-orange and finally to dingy yellow. The cap color starts off as dark red-bay brown with a dark olive-brown bloom, e.g., obs 248578, but then fades over time and can deteriorate to the color in the present obsie. Bluing of the elements goes without saying, of course.
As I mentioned in 255442, the LSU of 247957/255442/248580 doesn’t match the only B. subluridellus voucher in GenBank, KF030249 (<97% similarity). There is always going to be room for doubt in situations when species epithets cannot be anchored genetically because the holotype is too old for sequencing.

By: Geoff Balme (geoff balme)
2017-05-03 19:25:00 PDT (-0700)

are we thinking it’s subluridellus?

Discussion of Sequencing Results
By: I. G. Safonov (IGSafonov)
2017-05-03 18:31:53 PDT (-0700)

> A clean and contiguous nrLSU sequence of 966 bps was obtained from this collection and posted in the comment below.
> Alignment of this sequence with LSU sequences of other red-pored bolete collections in my possession revealed that it was identical to obs 255442 (also collected by Geoff at Umstead S.P.) and obs 248580 collected by Dario Z. in VA. I think all three are the same species.

That is GREAT!
By: Geoff Balme (geoff balme)
2016-11-07 14:11:29 PST (-0800)

I feel lucky to be of service here. And I’ll be very interested in the results!

Thanks, Geoff
By: I. G. Safonov (IGSafonov)
2016-11-07 14:05:22 PST (-0800)

I am going over the collections you sent me and cataloging them. Lots of interesting material! This one, like many others, will get sequenced.

By: Geoff Balme (geoff balme)
2016-11-07 13:16:40 PST (-0800)

hehehe stinking unidentifiable mushrooms.

By: I. G. Safonov (IGSafonov)
2016-11-07 13:12:45 PST (-0800)

Could you please make the third pic the thumbnail for this obsie? IMO it’s gives a better morphological overview of this interesting collection. Thanks.

By: Geoff Balme (geoff balme)
2016-08-15 12:49:03 PDT (-0700)

It looks also like a faded B. subluridus you’re right! I got excited about the B. subvelutipes but we could be between them here. That Yellow rim seems distinctive.

It keys to it if you decide
By: Geoff Balme (geoff balme)
2016-08-15 12:35:13 PDT (-0700)

the pore surface is Orange shade rather than Yellow shade key B-3 sect. 3a

KOH test fits too — maroon on the pileus. So maybe.

I just looked at
By: Geoff Balme (geoff balme)
2016-08-15 12:27:49 PDT (-0700)

a B. subvelutipes that looks good from Baker — MO #245367

These look like…
By: I. G. Safonov (IGSafonov)
2016-08-15 12:24:53 PDT (-0700)

…faded B. subluridellus. Boletus sensibilis is another somewhat far-fetched idea (I don’t see any vivid blue surface staining).
The all yellow (and pale at that) stipes are not consistent with L. carminipes. As a matter of fact the species name suggests what the stipe color should look like.

Maybe I should be checking
By: Geoff Balme (geoff balme)
2016-08-15 12:23:00 PDT (-0700)

a more light Orange “at first” pore surface.

The youngest specimen
By: Geoff Balme (geoff balme)
2016-08-15 12:16:21 PDT (-0700)

is certainly a pinkish-brownish yellow. The older ones are more yellow-slightly olive yellow.

From what I’ve seen that young specimen can’t possibly fit the zeitgeist. It is NOT yellow enough. Are there Pink-yellow pore surface possibilities?

By: I. G. Safonov (IGSafonov)
2016-08-15 11:59:00 PDT (-0700)

What’s the color of the pore surface? I cannot make an accurate assessment of it from your pix.