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21% (3)
Recognized by sight: Pale brown cap with striate margin, thick large white volval sac staining orangish, on ground under spruce, fir, pine and aspen at about 8300’ elevation
-42% (5)
Recognized by sight: see comments below
-6% (4)
Recognized by sight
ret
94% (3)
Based on chemical features: See comments, below

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= Observer’s choice
= Current consensus

Comments

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Thanks Rod!
By: Terri Clements/Donna Fulton (pinonbistro)
2019-05-17 16:40:18 PDT (-0700)

Very exciting news! I love the dark ring on the cap—present in both MO collections and Dr. States as well I think from your comments.
Terri

The LSU sequences provides a 100% match to a previous sequenc from castellae_.
By: R. E. Tulloss (ret)
2019-05-17 10:57:39 PDT (-0700)

The California collection to which I first appled the provisional name.

Synonymy.

Very best,

Rod

The sole previous collection of castellae is reported in MO #190682.
By: R. E. Tulloss (ret)
2019-05-17 10:28:55 PDT (-0700)

The images of castellae show a dark ring over the inner ends of the pileal marginal striations.

Hence, even without the nrLSU, we have visual evidence that sp-AZ28 (a collection made by Dr. Jack States) and A. castellae share at least one morphological trait.

The differences previously reported look like they could be two ambiguities and a SNP. I’m moving toward considering the temporary code and the provisional name synonymous.

This collection looks like it will make a difference before we even get all the molecular data. :)

R

We are beginning to process about 380+/- new reads from NAMP-Purdue.
By: R. E. Tulloss (ret)
2019-05-17 10:19:24 PDT (-0700)

For this voucher we have sequences for 202 characters from the lefthand (5’) end of nrITS (and including a smidgeon of the the terminal characters of the Small Subunit gene. This identifies the material as belonging in the “penetratrix group” of section Vaginatae. The best match in GenBank is to Amanitasp-AZ29”.

We also have a bit of the other end of nrITS for this material (202 characters). A BLAST run provides supporting evidence for the hypothesis states above. Moreover, we can see the characteristic Large Subunit 5’ motif for the group in question: TCTGACCTCAAATCA. The match (relying almost entirely on the 175 characters of LSU in the fragment) is to Amanita castellae.

Hopefully, an nrLSU sequence will appear from the reads we have not yet seen.

Since we are using two fragments we don’t have concurrence on a species epithet.

From the little we know of the two species, we have to compare them molecularly using nrLSU: The genetic distance is small—-0.22% in about 1400 characters (3 characters).

More when we have more.

Very best,

Rod

No sequences yet?
By: Alan Rockefeller (Alan Rockefeller)
2019-03-22 15:22:48 PDT (-0700)

Sure would be interesting if there was one.

not sure why this popped.
By: Debbie Viess (amanitarita)
2018-01-08 09:35:47 PST (-0800)

has this been examined and placed into a section, at least?

Igor
By: Debbie Viess (amanitarita)
2017-04-06 10:21:42 PDT (-0700)

I suspect that this is indeed a caesar’s mushroom. I can easily picture the extra material outside of that lower stipe to be veil material, which would result in a straight hollow stipe, thick membranous volva and a strongly striate cap margin.

That eventual DNA will get us to section, at least.

Nice find. There are several lifetimes of work in the grisettes. I look forward to those future publications and keys, which hopefully will talk about macro characters as well as micro and DNA!

Herbarium Rooseveltensis Amanitarum (R. E. Tulloss) has received specimen.
By: mcmacher
2017-04-06 09:50:59 PDT (-0700)

We have received the dried specimen. Thank you. It is being accessioned in Rod’s herbarium.

Section alternatives
By: I. G. Safonov (IGSafonov)
2017-03-16 18:39:19 PDT (-0700)

That’s a very unusual limbate-type volva for sect. Amanita. Admittedly, it does appear to have a bulb — at the very least the stipe doesn’t look like it’s totally elongating. If that feature is real, then it trumps the shape of volva characteristic of other sections. Amanita pudica from Africa is one such example sporting mixed morphology. Rod, do you think sect. Caesareae should still be considered?

Terri and Donna,
By: R. E. Tulloss (ret)
2016-08-17 04:29:40 PDT (-0700)

Thank you once again.

Very best,

Rod

Ok, will send it off with the others
By: Terri Clements/Donna Fulton (pinonbistro)
2016-08-16 21:29:41 PDT (-0700)
I think it will have inamyloid spores.
By: R. E. Tulloss (ret)
2016-08-16 21:20:32 PDT (-0700)

It looks like the volva contains a bulba at the stipe base. That would place it in section Amanita. The section doesn’t appear to reveal a totally elongating stem. I hope we can get DNA.

Thank you I would like to see what Linas can do with this.

Very best,

Rod

Yes, I noticed what appeared to be a small ring—
By: Terri Clements/Donna Fulton (pinonbistro)
2016-08-16 21:08:16 PDT (-0700)

hoped the photo would show it. So what section would this be in? I know it is immature but would you like a sample?

Terri

A very interesting species.
By: R. E. Tulloss (ret)
2016-08-16 20:53:15 PDT (-0700)

It appears their is a small ring on the stem and the volva seems completely connected to the stem’s base. A ring around the inner end of the marginal striations. I’m pretty sure this is a new one on me.

Fascinating.

Very best,

Rod