Collection location: Brendan T. Byrne State Forest, Burlington Co., New Jersey, USA [Click for map]
Project: Northeast Bolete Consortium
Several (3-4) collections of a total ~18 fruiting bodies at different stages of development made at the NJMA foray. Specimens growing in rich, moist and disturbed soil in pure stands of Atlantic white cedar. Two collections were made on the Batona Trail hugging Pakim Pond.
This is a very rare bolete (it should probably be added to the Red List of protected species as the habitat itself is endangered) that apparently has never been officially reported from NJ. The reported range of distribution in NAB/BRB is FL, GA and MA.
DNA Sequencing Data & Discussion (25-Jan-17):
> A clean, contiguous, 1263-nucleotide long nrLSU sequence was obtained for this material. Unfortunately, the first ~177 nucleotides from the 5’ end are missing (likely to be a QC issue on the sequencing company end). The missing region includes the LR0R, ITS4 and ITS6R primers that together account for 56 nucleotides. However, it’s still a usable sequence.
> A GenBank BLAST search of the full-length sequenced did not return any meaningful hits, and all the results had <97% similarity.
> Unsurprisingly, A BLAST search of a sequence fragment (the first 800 characters) did not return any close matches either. As a matter of fact the top scoring hit is only 98.3% similar. Interestingly, the first 7 top-scoring hits are all members of Chalciporus (97.5-98.3% similarity). The next 10 hits are a mixture of Chalciporus (5), Rubroboletus (3), and Butyriboletus (2).
Discussion (25-Jan-17): While B. purpureorubellus is unlikely to be a member of Chalciporus based on morphology alone, the GenBank BLAST search results suggest this unusual bolete could indeed be falling closer to Chalciporus on the family phylogenetic tree than to any other genus in the Boleteaceae. The subfamily Chaciporoideae, comprised of Chaciporus and Buchwaldoboletus, happens to be the basal clade in Boleteaceae, meaning that it’s “the earliest branching lineage” of boletes (Nuhn et al., 2013, and Wu et al., 2014 & 2016). The pseudo-boletinoid pore surface and the possibly non-mycorrhizal lifestyle of B. purpureorubellus could also be the supporting manifestations of the early diverging origin of this taxon. Could the small spore size be a link with Buchwaldoboletus?
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sum(score * weight) /
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Unfortunately, I didn’t subject this or the earlier collection to treatment with chemicals. I think I did save the fb with the yellow stipe as it was one of the freshest in this voucher.
I’ve got to sample this critter for another locus, a single-copy gene. I am hopeful it will deliver a better phylogenetic inference than LSU. Presumably Dr. Kudzma still has some extracted DNA stock stored away. I would hate to find out my dried voucher is out of DNA even though it was stored all this time under the best possible conditions (in a fridge).
Igor did you record chem data on this and your other collection? I’m interested in the fruiting body that has a mostly yellow stipe. I am used to seeing it with much more red for the entire length of stipe.
I was very excited about this unique find. I tried to key it out in the bolete book, but failed. Apparently I was looking at the wrong key – smooth & textured stipes instead of reticulated stipes (the apex reported as slightly reticulated)! I was flipping through the photographs in the book and only then made the connection. Also, I just realized obs 248331 from August was also B. purpureorubellus. Needless to say, I failed to find in the book back then!
I will look for that article.
The area I found is spruce flats bog. There is a paper on the Atlantic white cedar there – Natural Areas Journal Volume 29 issue 3 2009 – Current Status and Future Development of the Only Chamaecyparis thyoides (Atlantic White-Cedar) Population in Pennsylvania
Where is the PA stand of White Cedar that you are thinking of? I know of a few trees planted in Philadelphia, but nowhere else in the state.
I am so happy you got to find this cool bolete. I just checked to see if we have any atlantic white cedar here in PA. There is only one known location. I will be checking it this weekend. It was planted in the 40s.