Observation 281329: Boletus vermiculosoides A.H. Sm. & Thiers
When: 2017-07-08
Collection location: Lynn Valley Rd. and Haldimand Rd. 70, Port Dover, Ontario, Canada [Click for map]
Who: Eva Skific (Evica)
Specimen available
Fungarium record:
Species Lists
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| User’s votes are weighted by their contribution to the site (log10 contribution). In addition, the user who created the observation gets an extra vote. | |||||||||
| Vote | Score | Weight | Users | ||||||
|---|---|---|---|---|---|---|---|---|---|
| I’d Call It That | 3.0 | 0.00 | 0 | ||||||
| Promising | 2.0 | 6.97 | 1 | (Evica) | |||||
| Could Be | 1.0 | 0.00 | 0 | ||||||
| Doubtful | -1.0 | 5.13 | 1 | (IGSafonov) | |||||
| Not Likely | -2.0 | 0.00 | 0 | ||||||
| As If! | -3.0 | 0.00 | 0 | ||||||
| Overall Score sum(score * weight) / (total weight + 1) |
0.67 | 22.41% | |||||||
| User’s votes are weighted by their contribution to the site (log10 contribution). In addition, the user who created the observation gets an extra vote. | |||||||||
| Vote | Score | Weight | Users | ||||||
|---|---|---|---|---|---|---|---|---|---|
| I’d Call It That | 3.0 | 0.00 | 0 | ||||||
| Promising | 2.0 | 5.13 | 1 | (IGSafonov) | |||||
| Could Be | 1.0 | 0.00 | 0 | ||||||
| Doubtful | -1.0 | 0.00 | 0 | ||||||
| Not Likely | -2.0 | 0.00 | 0 | ||||||
| As If! | -3.0 | 0.00 | 0 | ||||||
| Overall Score sum(score * weight) / (total weight + 1) |
1.67 | 55.79% | |||||||
Comments
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Thank you, Eva
By: I. G. Safonov (IGSafonov)
2017-07-08 17:40:22 -05 (-0500)
Yes, I would be interested in a sample.
Cool bolete, Eva
By: I. G. Safonov (IGSafonov)
2017-07-08 17:26:46 -05 (-0500)
Do you think the pore surface is originally brown and fading to dingy yellow, or the other way around? Have you preserved it?
> A clean and contiguous nrDNA sequence of 1687 bps was procured from this material. The nrITS portion consists of the last 707 bases; the nrLSU portion consists of the first 980 bases (through to the LR5 region). There are no ambiguous characters, so it’s a single haplotype for both loci.
> This nrLSU sequence is 99.9% similar to the corresponding fragment in the LSU of obs 210721 and 100% identical to the LSU of obs 279688. This empirical results suggests conspecificity of the three collections. For a discussion of pertinent BLASTn results see obs 210721.
As it has been stated by me a couple of time elsewhere on MO, the LSU sequencing effort of red-pored collections from the eastern/northeastern North America (SE Canada south to NC) so far identified 9 unique genotypes, representing at least as many morphological species, that are likely residents of the prolific Neoboletus-Sutorius association in the sense of Wu et al.1,2
> A BLASTn search of the nrITS sequence returned a hit list that is indicative of this taxon being part of a very morphologically and genetically diverse Neoboletus-Sutorius association. In particular, the top 4 hits are boletes from Canada collected by Renee Lebeuf and Yves Lamoureux (list sorted by max score):
> KY826093: Boletales sp. voucher B2842 = 706/707 = ~99.9% similar (no pic available)
> KY826098: Boletales sp. voucher B2858 = 705/708 = 99.6% similar (see pic at https://www.flickr.com/...)
> KY826193: Boletales sp. voucher RL042 = 691/692 = ~99.9% similar (see pic at https://www.flickr.com/...)
> KY826168: Boletales sp. voucher RL013; 690/692 = 99.7% similar (see pic at https://www.flickr.com/...)
Also, the last 542 characters of this nrITS sequence are a 99.9% match (1 base off) to the nrITS sequence of obs 279688.
References:
1 “Molecular Phylogenetic Analyses Redefine Seven Major Clades and Reveal 22 New Generic Clades in the Fungal Family Boletaceae”, Fungal Diversity 2014, 69(1), pp. 93-115.
2 “One Hundred Noteworthy Boletes From China”, Fungal Diversity 2016, 81, pp.25-188.