Notes:
Pine, oak, cypress.

Images

IMG_20180117_124720.jpg
1000x1s.jpg
1000x2s.jpg
IMG_20180117_124701.jpg
IMG_20180117_124709.jpg

Proposed Names

ret
82% (1)
Based on chemical features: combined nrITS-LSU was derived from this material.

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= Observer’s choice
= Current consensus

Comments

Add Comment
More data.
By: R. E. Tulloss (ret)
2019-12-02 18:29:06 CST (-0500)

There are about twice as many sequences for subnigra as there are sequences for lindgreniana in my local data base. In subnigra, the extant sequences show no variation at all. Zip. Where lindgreniana shows two possible values at a character position, __subnigra_ sticks to one of the two possibilites with no hint of anything else.

That’s all I’m going to do today on this issue.

R.

I will email you an alignment showing a segment of the ITS2 region of nrITS.
By: R. E. Tulloss (ret)
2019-12-02 18:05:39 CST (-0500)

This is the only area in which the sequences from California and Washington show variation. These three characters positions contain 2 fairly clear SNPs and one possible SNP. I would argue that the grounds are supportive of treating them all as one species barring other evidence from another source or sources. To give your reference points in the image, the ITS2 region begins at character 388. The nrITS gene (and its ITS2 component) end at character position 545. The three sequences in the comparison differ nowhere other than the three character positions in the photo.

So, both the nrITS and nrLSU sequences support the hypothesis that Amanita lindgreniana has a range extending fro Washington to central California.

R

R

The strong match from the BLAST run is very dependent on the similarity of the nrLSU…
By: R. E. Tulloss (ret)
2019-12-02 16:00:20 CST (-0500)

…sequences. The overlap region treated by BLAST only contained a few characters of nrITS. So I will go further with analysis of the sequence results. With regard to the nrLSU on two differences in the BLAST results are due to a straight forward conflict of non-ambiguous characters - 2 characters out of 1459 in the overlap region. I think we need to take a look at the nrITS comparison that we can make. That means I have to segregate an ITS sequence on which to make the comparison.

I’m being taken for a walk, now.

This will take time.

Very best,

Rod

Hi Rod
By: banders (banders)
2019-12-02 15:58:18 CST (-0500)

Looking back at pictures from that day, that was the only fruit body I saw so I can’t comment on gill color. It was growing under Pinus radiata and Quercus agrifolia. There are some eucalyptus and Cupressus macrocarpa around as well.

Hello, Ben.
By: R. E. Tulloss (ret)
2019-12-02 15:46:02 CST (-0500)

Do you recall the gills as being so strongly non-white?

Do you know the botanical name of the cedar(s) near the collecting site of this species?

Thanks for sending this interesting species.

R

We have successfully obtained a sequence which BLASTs to a very good match to our…
By: R. E. Tulloss (ret)
2019-12-02 15:43:42 CST (-0500)

…earlier sequences for Amanita lindgreniana. The cap appears to have a much grayer appearance in comparison to previous images of the species that show more brown in the pigmentation. Since we have very few collections and are still looking for photos, we can’t be sure what to say about pigmentation of this species. Examination of additional genes would be a good project.

Very best,

Rod

This material has been accessioned. The accession no. is RET “822-4.”
By: R. E. Tulloss (ret)
2018-07-11 16:01:54 CDT (-0400)

Thank you very much for sending us this interesting material.

Very best,

Rod

Many thanks.
By: R. E. Tulloss (ret)
2018-01-18 18:40:52 CST (-0500)

I look forward to the shipment of goodies.

Very best,

Rod

Hi Rod,
By: banders (banders)
2018-01-18 18:32:29 CST (-0500)

Yes, I’ll add it to what I’m sending you.

Do you have dried material?
By: R. E. Tulloss (ret)
2018-01-18 18:18:14 CST (-0500)

Very best,

Rod Tulloss