Notes:
Very little KOH reaction, always has a yellowish cap center. Under oak.

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Rod,
By: I. G. Safonov (IGSafonov)
2020-06-30 18:05:44 CDT (-0400)

Have you tried BLASTn-ing a shorter LSU fragment between LSU0R and LR5 primers (800-900 bps)? In my experience BLASTn is very sensitive to query length — when long sequences are run, BLASTn often returns less similar hits of comparable lengths rather than shorter but more similar ones. There majority of LSU sequences in GB don’t go beyond the LR5 area.
How does the LSU sequence compare with that of obs 370788? I understand, however, from the extensive discussion in that post that such a comparison could be meaningless.

Well, one problem is that this doesn’t appear to be a species section Phalloideae.
By: R. E. Tulloss (ret)
2020-06-30 14:33:10 CDT (-0400)

In Bas’ taxonoomy this would be in subsection Limbatulae.

In Cui et al.’ taxonomy this would be in section Roanokenses.

I was able to get sequence roughly positioned near the 5’ end of the LR0R forward read and roughly near the 3’ end of the reversed LR6 read. Neither had a closet BLAST match under 1% genetic distance. However, the closest match for one was Amanitasp-C05” and for the other the closest match was the notorious Amanita oberwinklerana. I believe that in both cases the nearest match would be in the position described at the outset of this comment.

It is probably hard to get an nrITS sequence from something of the general grouping to which this belongs.

Anyway, part of the puzzle is that this is not a species of the Phalloideae.

Very best,

Rod

Created: 2018-11-09 23:53:55 CST (-0500)
Last modified: 2020-06-30 18:05:45 CDT (-0400)
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