Observation 352633: Bisporella citrina (Batsch) Korf & S.E. Carp.

Notes:
Growing on a hardwood stick. Paraphyses with swollen tips. Asci unitunicate, inoperculate and 8-spored. Spores inamyloid, smooth and thin-walled, some becoming 1-septate. Spore measurements from Piximetre: (9.3) 9.9 – 13.8 (16.1) × (3) 3.3 – 4.2 (4.5) µm, Q = (2.3) 2.7 – 3.7 (4.5); N = 30, Me = 11.9 × 3.7 µm; Qe = 3.2
Individual spores: 11.05 × 4.49 µm, 11.95 × 4.35 µm, 13.01 × 4.22 µm, 12.09 × 3.91 µm, 12.00 × 3.28 µm, 11.96 × 3.94 µm, 12.70 × 3.70 µm, 9.65 × 3.71 µm, 11.31 × 3.93 µm, 12.53 × 3.56 µm, 14.32 × 3.19 µm, 12.08 × 3.23 µm, 13.10 × 3.76 µm, 10.67 × 3.65 µm, 10.36 × 3.68 µm, 12.09 × 3.64 µm, 16.12 × 3.89 µm, 13.79 × 3.86 µm, 9.85 × 3.49 µm, 11.76 × 4.42 µm, 13.76 × 3.53 µm, 9.69 × 4.17 µm, 11.01 × 3.82 µm, 13.32 × 3.71 µm, 9.86 × 3.04 µm, 9.33 × 3.40 µm, 12.13 × 3.34 µm, 13.19 × 3.91 µm, 10.74 × 3.73 µm, 12.38 × 3.42 µm

Species Lists

Images

2018-12-27-01.10.52 ZS DMap edit.jpg
20181225_132305.jpg
20181225_132257.jpg
DSC_7973 Outer surface and spores at 400x in Melzer's.JPG
DSC_7975 Outer surface and spores at 400x in Melzer's.JPG
DSC_7988 Spores at 400x in Melzer's.JPG
DSC_8002 Asci, paraphyses and spores at 400x in Melzer's.JPG
DSC_8006 Asci, paraphyses and spores at 400x in Melzer's.JPG

Proposed Names

29% (1)
Recognized by sight
61% (2)
Used references: Dennis, R. W. G. (1978). British ascomycetes. J. Cramer.
Seaver, F. J. (1951). The North American Cup-fungi (Inoperculates). The North American cup-fungi (Inoperculates).
Kuo, M. (2008, October). Bisporella citrina. Retrieved from the MushroomExpert.Com Web site: http://www.mushroomexpert.com/bisporella_citrina.html
Based on microscopic features

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= Observer’s choice
= Current consensus

Comments

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My mistake
By: Django Grootmyers (heelsplitter)
2019-03-02 12:53:32 MST (-0700)

I don’t think the asci are shown clearly enough to make that call here. Should have probably looked at them at 1000×. Regardless, this keyed out to Bisporella citrina in the references listed without taking the amyloidity of the asci into account.

I have recently acquired some fresher Melzer’s reagent, but this Melzer’s worked fine despite being from the ‘70s. I tested it with 2 positive controls and a negative control before using it further. The issue with it was that someone in my lab had accidentally gotten some thick-walled conidia in the Melzer’s bottle so it was annoying to work with.

if all tissues are IKI-
By: Danny Newman (myxomop)
2019-03-02 10:11:10 MST (-0700)

this can’t be Bisporella at all, much less B. citrina. there ought to be an amyloid apical apparatus. check your melzer’s?

Created: 2018-12-25 22:10:32 MST (-0700)
Last modified: 2019-05-15 19:24:37 MDT (-0600)
Viewed: 42 times, last viewed: 2019-05-16 09:19:31 MDT (-0600)
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