Observation 97281: Corticiaceae sensu lato

On an archway built by my father.

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Thank you both!
By: Tim Sage (NMNR)
2012-06-14 11:06:06 PDT (-0700)
section or pluck
By: else
2012-06-14 10:44:55 PDT (-0700)

you indeed take a bit from the hymenium, or section (if it is a tougher specimen, like the one shown here).
the real corticioid-specialists predominantly work with dried material, actually.
and you look for the normal structures, as i said, basidia, cystidia, kind of hyphae.
if there are spores, there should be at least basidia.

Any tips on how to mount?
By: Douglas Smith (douglas)
2012-06-14 10:24:25 PDT (-0700)

Ok, sure, wait for it at least to make spores. But once you decide to mount it on a slide, how do you section it? For an agaric, I know I need to make a mount of a gill, some section of the cap surface (usually a radial section), and a section of the upper (and even lower) stipe for the gold star. But when I’m just looking at a crust, I grab my double-edged-razor blade, and then I wonder, well what do I do now?

I kinda guess I want to get some amount of the hymenium, and not get any woody surface in the mount if possible… Do I just pinch a bit of hymenium with the forceps and just squash it, and see what happens? Thats what I’ve done so far, and most the time I’m just looking at a mass of random hyphae with no structure, except maybe clamps…

what to do with corticioid fungi
By: else
2012-06-14 09:46:30 PDT (-0700)

first you make a spore print – put the fruiting body with the spore bearing surface down on a microscope slide, wrap everything in a wet paper towel, put it in a wax paper bag or a plastic bag and let it sporulate.

next day, inspect the slide, if there are spores keep the specimen, if not, throw it out, and go on with your life.

check whether the spores have a reaction in Melzer’s reagent.

get the big books out and identify. look for cystidia, basidia, clamp connections, the usual, they are not that different from gilled mushrooms.

What should I look at?
By: Tim Sage (NMNR)
2012-06-14 03:19:06 PDT (-0700)

Any tips for microscopy?

Created: 2012-06-13 16:35:27 PDT (-0700)
Last modified: 2012-08-31 14:25:51 PDT (-0700)
Viewed: 102 times, last viewed: 2019-02-24 12:58:08 PST (-0800)
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