The aim of a crush mount is to get cells to break free so that each entire cell can be viewed (whether it’s for viewing a basidium, a cheilocystidium, a pleurocystidium, etc). This is performed after applying the cover slip.
A. Put the best material available on a slide with an appropriate amount of KOH solution. Other liquids work for this too. Then add a cover slip.
B. Get a pencil with an eraser. Press down with the pencil eraser end onto the cover slip where the tissue sample lies.
C. Gently, repetitively flatten the tissue and try to eliminate any air bubbles.
The goal is to free up the cells (basidia, cystidia, etc) so they can be viewed with better visibility. You have to utilize the appropriate amount of pressure to get good visibility but not so much that you destroy the sample. It can take several attempts and requires a bit of patience.
Too little pressure and the cells are not freed.
Too much and the cystidia are crushed.
Note: It helps to cover your finger with a kimwipe/tissue/paper towel and press down semi-hard on the cover slip.
Tip: The squashing/crushing process with the eraser forces extra water out from under the cover slip and can sometimes rise onto the cover slip. Use napkins to soak this water during the squashing process.
Created: 2019-07-20 06:09:05 CDT (-0400)
Last modified: 2019-08-05 21:13:19 CDT (-0400)