Glossary Term: PCR Inhibition

(Polymerase Chain Reaction Inhibitors)

PCR inhibitors are any factor which prevent the amplification of nucleic acids through the polymerase chain reaction (PCR). PCR inhibition is the most common cause of amplification failure when sufficient copies of DNA are present. PCR inhibitors usually affect PCR through interaction with DNA or interference with the DNA polymerase. Inhibitors can escape removal during the DNA purification procedure by binding directly to single or double-stranded DNA.

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Created: 2019-06-27 06:05:33 EDT (-0400)
Last modified: 2019-08-18 14:38:53 EDT (-0400)