Draft of Grosmannia clavigera (Rob.-Jeffr. & R.W. Davidson) Zipfel, Z.W. de Beer & M.J. Wingf. for 2010/2011 EOL University Species Pages Initiative by Chris Anderson

Title: Draft For 2010/2011 Eol University Species Pages Initiative By Chris Anderson (Public)
Name: Grosmannia clavigera (Rob.-Jeffr. & R.W. Davidson) Zipfel, Z.W. de Beer & M.J. Wingf.
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 Draft For 2010/2011 Eol University Species Pages Initiative By Chris Anderson (Public)

Description status: Approved
 (Latest review: 2010-12-17 10:27:25 CST (-0600) by Anne Pringle)

Taxonomic Classification:

Domain: Eukarya
Kingdom: Fungi
Phylum: Ascomycota
Class: Sordariomycetes
Order: Ophiostomatales
Family: Ophiostomataceae


General Description:

Introduction

Grosmannia clavigera is a widespread pathogen of North American pine trees and a common (although not obligate) symbiont of bark beetles (family: scolytidae) of the genus Dendroctonus. G. clavigera is known as a bluestain fungus because of the characteristic blue-gray stain that it gives to infected wood. Over the course of an infection, G. clavigera spreads throughout the wood of the host tree. If left unchecked, G. clavigera infections will eventually disrupt the transport of nutrients and water which will result in the death of the host tree. G. clavigera can also prevent the flow of sap, which drastically lowers the host tree’s resistance to infestation from bark beetles, who in turn, carry spores and help propagate the fungus.

General Biology

G. clavigera is an ascomycete and as such, is characterized by having ascii that produce and discharge a special type of spore known as an ascospore. The assimilative phase of G. clavigera is spent in the phloem and sapwood of a host pine tree is and characterized by a large, blue mycellium. The cleistothecia are small (5.5-9.0 microns in diameter) but are grouped together in circular arrangements on sapwood with a diameter of 250-640 microns. The cleistothecia are globose, with a black and leathery exterior. Spore discharge occurs through an irregular rupture in the cliestothecium wall. After discharge, the cleistothecia are concave and saucer like. Each ascus produces eight ascospores, which are unicellular and semicircular in appearance. Since the primary mode of dispersal for G. clavigera is through spore adhesion to beetle symbionts, the ascospores are very sticky. This “stickyness” might also contribute to the fungus’ ability to stop nutrient transport in host trees by “plugging up” critical transport structures.

As a Pathogen

G. clavigera is an important pathogen of North American pine trees, specifically lodgepole pine (Pinus contorta), whitebark pine (P. albicaulis), ponderosa pine (P. ponderosa), western white pine (P. monticola), and limber pine (P. flexilis). A tree infected with G. clavigera shows relatively few signs or symptoms, although it is sometimes possible to notice large, blue-gray lesions underneath bark. The only sure-fire way to accurately identify whether or not a tree has been infected by a bluestain fungus is to examine the phloem and sapwood, which is typically only possible after the tree has already died.

Although G. clavigera can infect a wide variety of hosts, it is especially virulent in lodgepole pine. It is particularly lethal to seedlings although it has been known to kill mature lodgepole pines in less than a year after inoculation. The mechanism by which G. clavigera kills trees is thought to be a combination of its ability to prevent both nutrient transport in the phloem and the production of sap in the sapwood. The first mechanism is straight-forward in its lethality: if the tree can’t distribute nutrients, it will die. The latter mechanism, however, is usually only fatal when the fungal infection occurs simultaneously with a bark beetle infestation.

Another factor which adds to G. Clavigera’s virulence is the fact that it seems to be adept at colonizing otherwise healthy trees. This is in stark contrast with similar, closely-related fungi which are only found in trees that are already unhealthy.

As a Symbiont

G. clavigera can participate in several symbiotic relationships that aren’t directly pathogenic. The most striking relationship that exists—the one that is responsible for the great success of __G. clavigera in North America—is between G. clavigera and the bark beetle, Dendroctonus ponderosae. Since the bark beetles are interested in colonizing and laying their eggs in the same trees that G. clavigera is, these two organisms have developed a mutualistic relationship where both organisms receive some sort of benefit. The fungus receives a benefit from the bark beetles in that its spores can be carried safely and reliably between trees by bark beetles. Also, once again, since the symbiotic relationship between G. clavigera and the bark beetles is mutualistic, there are several advantages that the fungus confers to its bark beetle symbionts. As has been mentioned earlier, G. clavigera can help to exhaust the defenses of the host trees, which makes it much easier for bark beetle infestations to take hold. In addition to this, however, it has also been shown that G. clavigera can be eaten by the bark beetles and contains essential nutrients which increases the brood size and viability of the bark beetles. In an interesting complication, it has also been shown that G. clavigera shares an additional beneficial relationship with symbiotic bacteria that live on the bark beetles. In the presence of these symbiotic bacteria, spore production was greatly stimulated in G. clavigera.

These relationships are important because they are apparently a contributing factor to the virulence of G. clavigera and the damage potential of D. ponderosae. When either the bark beetles or fungus colonizes a tree on its own, the host tree has a reasonable chance of survival. When G. clavigera and D. ponderosae colonize a tree at the same time, however, it is almost assured that the tree will die.


Diagnostic Description:

G. clavigera closely resembles several ophiosomatoid fungi and can only be reliably distinguished through genetic analysis. Despite this, there are a couple of characters that can help you, very roughly, in indentifying G. clavigera:

The spores of G. clavigera are semicircular whereas the spores of fungi in Ceratocystiopsis and Europhium are sickle-shaped and hat-shaped, respectively.

Also, since G. clavigera is more virulent than other, closely related taxa, it might be possible to distinguish an outbreak of G. clavigera by the rapidity in which infected trees die.


Distribution:

G. clavigera is found from Mexico to Canada along the Rocky Mountain range, although there is concern that the warming climate will cause G. Clavigera’s range to expand further northward into the lower altitude forests of northern Canada.


Habitat:

G. clavigera grows best when the temperature is around 20-25C but can survive temperatures as low as -20C. Any temperatures higher than 30C will kill G. clavigera. Because of these temperature constraints G. clavigera is typically found in sub-Alpine coniferous forests where the temperature is relatively cool all year round.

G. clavigera’s habitat is quickly shifting, however, due to warming climates. The past several years have seen an increase of habitat types that G. clavigera can inhabit as its range pushes higher in altitude and further north.


Look Alikes:

There are several other bluestain fungi that have associations with bark beetles but are genetically distinct. The lookalikes are generally in the genus Ophiostoma and include (but are not limited to) Ophiostoma huntii, Ophiostoma montium, and Leptographium longiclavatum.


Uses:

Since G. clavigera is a deadly tree pathogen, much of the attention that has been paid to it has been directed toward its eradication. In recent years, however, there has a growing trend among regional Forest Services of “making the best of a bad situation” by selling blue-stained wood at premium prices. The color that G. clavigera stains wood is generally thought to be aesthetically pleasing, and as such, wood that is harvested from fungus and beetle-killed trees are more valuable than wood from their more healthy brethren. The Colorado Forest Service (a state hit particularly hard by G. clavigera and its beetle symbiont), for instance, has a program which allows, for a premium, lumber companies to harvest trees that have been killed by the bluestain fungus on Forest Service land. This program, in turn, generates funding for several campaigns that are directed toward the control of both G. clavigera and D. ponderosae.


References:

Journal Papers

A.S. Adams, C.R. Currie, Y. Cardoza, K.D. Klepzig, and K.F. Raffa. (2009) Effects of symbiotic bacteria and tree chemistry on the growth and reproduction of bark beetle fungal symbionts. Can. J. For. Res. 39: 1133–1147

Christiansen, E., Solheim,H. (2007) The bark beetle-associated blue-stain fungus Ophiostoma polonicum can kill various spruces and Douglas fir. Journal of Forest Pathology. 20(6-7), 436-446

Jae-Jin Kim, Eric A. Allen, Leland M. Humble, and Colette Breuil. (2005) Ophiostomatoid and basidiomycetous fungi associated with green, red, and grey lodgepole pines after mountain pine beetle (Dendroctonus ponderosae) infestation
Canadian Journal of Forest Research 35: 274–284

Lee, S., Kim, J.-J., and Breuil, C. 2006. Diversity of fungi associated with the mountain pine beetle, Dendroctonus ponderosae, and infested lodgepole pines in British Columbia. Fungal Di-vers. 22: 91–105.

Owen, D.R.; Linuaiil, K.Q. Jr.; Woodd L.; Parme’l’lill, J.R.Jr.,(1987) Pathogenicity of fungi isolated from Dendroetonus valens, D. brcvicomis, and D. ponderosae to ponderosa pine seedlings. Phytopathology 77, 631-636

Rice A.V., Thormann M.N., and Langor D.W. (2007) Virulence of, and interactions among, mountain pine beetle associated blue-stain fungi on two pine species and their hybrids in Alberta. Canadian Journal of Botany 85: 316–323

Rice A.V., Thormann M.N., and Langor D.W. (2008) Mountain pine beetle-associated blue-stain fungi are differentially adapted to boreal temperatures. Forest Pathology 38: 113-123

Robinson-Jeffrey, R.C.; Davidson, R.W.(1968) Three new Europhium species with Verticicladiella imperfect states on blue-stained pine. Canadian Journal of Botany 46(12): 1523

Six, D.L.; Harrington, T.C.; Steimel, J.; McNew, D.; Paine, T.D. (2003) Rust fungi causing galls, witches’ brooms, and other abnormal plant growths in northwestern Argentina. Mycologia 95(5): 791

Six, D.L., and Paine, T.D. 1998. Effects of mycangial fungi and host tree species on progeny survival and emergence of Den- droctonus ponderosae (Coleoptera: Scolytidae). Environ. Ento- mol. 27: 1393–1401.

Y. Yamaoka; R. H. Swanson; Y. Hiratsuka. (1990)Inoculation of lodgepole pine with four blue-stain fungi associated with mountain pine beetle, monitored by a heat pulse velocity (HPV) instrument. Canadian Journal of Forest Research 20 pp 31-36

Y. Yamaoka, Y. Hiratsuka and P. J. Maruyama. (1995) The ability of Ophiostoma clavigerum to kill mature lodgepole-pine trees. European Journal of Forest Pathology 25: 401-404

Zipfel, R.D.; Beer, W. de; Jacobs, K.; Wingfield, B.D.; Wingfield, M.J. (2006) Multi-gene phylogenies define Ceratocystiopsis and Grosmannia distinct from Ophiostoma. Studies in Mycology 55: 90

Websites

http://www.coloradoforestproducts.org/bluewood.htm


Notes:

Taxonomic History

Grosmannia clavigera was first described in as Euphorium clavigera in 1968 but has since undergone several revisions. It has also been known as Ceratocystis clavigera and Ophiostoma clavigera. It was most recently revised in 2006 when molecular data suggested that G. clavigera was not a member of Ophiostoma, although it is closely related. Despite this rather messy history, the most recent revision of G. clavigera has been widely accepted and now appears to be standard.

Associated Anamorphs

Leptographium clavigerum is the only known anamorph of G. clavigera.


Description author: Chris Anderson (Request Authorship Credit)


Created: 2010-11-07 19:54:16 CST (-0600) by Chris Anderson (neverlupus89)
Last modified: 2011-07-29 19:03:48 CDT (-0500) by Chris Anderson (neverlupus89)
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