Observation 152692: Suillus Gray

When: 2013-11-16

Collection location: Franklin Parker Preserve, Chatsworth, New Jersey, USA [Click for map]

39.8143° -74.5479°

Who: I. G. Safonov (IGSafonov)

Specimen available

Notes:
A group of 6 medium-sized specimens growing in sandy soil under pitch pines. Three specimens in best shape have been photographed and preserved. Half of the dried material is in the hands of Dr. Alan E. Bessette.

Description:
> The caps are convex to sub-convex and somewhat wavy in shape, ≤6.5 cm in diameter; the margins are non-appendiculate, but possibly with some subtle sterile tissue present(see pix).
> The sticky, glutinous, peelable cuticle is brown to reddish-brown (the flash makes them look orange-brown) in color; areas of the cuticle below clumps of dirt/sand are pale yellow and occasionally streaked with green (see pix).
> The pore surface is brownish-buff, sub-decurrent, transitioning into a scruffy, pale yellow partial reticulum; the pores are small, circular to angular, ~2/mm on average, non-boletinoid and non-radially arranged (see pix).
> The stipes are the most curious feature of this mushroom. It’s pale yellow overall with patchy brownish stains (especially near the base), generally tapering downward and curved at the bottom, and terminating in a pointy, root-like projection; the upper portion (up to 1/3) is covered with raised, scruffy reticulum (one specimen was particularly reticulate, while others were less so) transitioning to minute scruffiness below (see pix); no partial veil/annulus or remnants thereof were present; no glandular dots/smears were observed.

Microscopic observations (documented by Dr. Bessette):
Spores (taken from the dried material) are 8-10(-11) x 3-4 microns (mounting medium unknown). Cystidia are rare, clavate, thin-walled and hyaline. Basidia are hyaline and 4-sterigmate. “Not mush else of significance”.

Macrochemical tests:
KOH = blackish on cuticle; brown on pores; flashing pink and instantly changing to lavender blue on context.
NH4OH = grayish on cuticle, pinkish-orange on pores, pinkish-red on context
FeSO4 = bluish-green on context.
The outcome of these tests is in good agreement with MO #82449. That mushroom, also collected in mid-November (albeit at a different location) and identified as S. brevipes by me, bears some resemblance to this post despite having a much shorter stipe and sporting glandular dots.

rDNA Studies:
The ITS region of rDNA was recently sequenced (711 nucleotides long) by a non-commercial lab in NJ. Alignment of the contiguous composite sequence (supported by both the reverse and forward reads) with the GenBank data yielded two highly similar sequences — both have 99% similarity, with only 1 gap in the same location at the very end of the sequence). The first sequence [Jones,M.D., Phillips,L.A., Treu,R., Ward,V. and Berch,S.M. Agric., Ecosyst. Environ., Appl. Soil Ecol. 60, 29-40 (2012)] was obtained from an environmental sample (a root tip with attached mycelium) and attributed to S. luteus. The second sequence [Kranabetter,J.M., Friesen,J., Gamiet,S. and Kroeger,P. Mycorrhiza 19 (8), 535-548 (2009)] came from a S. brevipes voucher SMI330. In both cases, the studied material was collected in British Columbia!!!

Species Lists

Images

385860
385861
385862
385863
385864
Reticulation pattern on stipe
400064
400065

Proposed Names

57% (1)
Eye3 Eyes3
Recognized by sight
46% (2)
Based on chemical features: GenBank BLASTn data

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

Add Comment
Thanks, Alan
By: I. G. Safonov (IGSafonov)
2017-12-31 18:27:57 CST (-0500)

Actually this happens to be the first or second sequence I ever obtained from any of my collections.
Yes, there are several ambiguous characters that amount to a large number of haplotype combinations within this organism, but, in my understanding, at least one of those copies is going to match a reliable homotypic brevipes example in GenBank.
My “analysis” in the notes here dates back to 2014, I haven’t really updated it since then, and at that time there were many fewer sequences in GenBank for BLASTing. There are more hits now, but the profile hasn’t really changed that much. I also recall contacting Dr. Bruns via email regarding this critter a couple of years ago. His response was that it’s as good as it gets with this taxon, perhaps suggesting it’s a cluster of species that ITS doesn’t resolve well.
IMO, this particular example is not a dead-ringer for brevipes gestalt. I collected what I believe is the textbook brevipes a couple of months ago, obs 297268. To me they look like different mushrooms. It don’t know if it’s worthwhile to look at another locus to probe this theory, as I can easily think of more interesting cases to spend my money on.
Lastly, thank you for removing those sequences from the three obsies I pointed out. I apologize for the harsh wording in my original comment.
Happy New Year!

Thanks, and I agree
By: Alan Rockefeller (Alan Rockefeller)
2017-12-31 17:32:25 CST (-0500)

Thanks for the sequence. There are 7 differences between this and the closest Suillus brevipes sequence in GenBank, but most of those differences are nucleic acid ambiguity codes, meaning this is probably S. brevipes.

Yes, I do
By: I. G. Safonov (IGSafonov)
2017-12-31 11:10:26 CST (-0500)

Will upload soon.

Do you have the sequence for this?
By: Alan Rockefeller (Alan Rockefeller)
2017-12-31 09:43:36 CST (-0500)

Created: 2013-11-16 22:59:45 CST (-0500)
Last modified: 2018-05-02 23:11:06 CDT (-0400)
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