Proposed Names

47% (2)
Recognized by sight
55% (1)
Based on chemical features: Based on DNA sequences described in comment below.
82% (1)
Based on chemical features: This collection provides the basis of the concept behind the temporary code number.

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= Observer’s choice
= Current consensus


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The GB accession numbers have been added.
By: R. E. Tulloss (ret)
2019-03-04 08:01:32 PST (-0800)


Today both nrITS and nrLSU for this material have been submitted to GenBank.
By: R. E. Tulloss (ret)
2019-02-28 11:52:25 PST (-0800)

It looks like there are one or more ambiguities near the interface between nrITS and nrLSU. We could not penetrate into that region in either direction (i.e. not from either of the nrITS related reads, nor from the relevant nrLSU-related read).

As more material similar to sp-C22 builds up in the herbarium, we will get more chances to see if the hypothesis of a region of heterogeneity is justified and whether the heterogeneity can be nailed down with future attempts using additional primers.

The next thing we’ll post here are a pair of GenBank accession numbers. :). Maybe next week.

Very best,


Genetic distance to calyptratoides
By: R. E. Tulloss (ret)
2015-01-03 11:43:06 PST (-0800)

For the nrLSU sequences, the difference (genetic distance) is manifested by differences in 4.3% of the characters in the region of overlap (our new sequences use different primers than Sanchez did and are several hundred characters longer). This is a substantial difference for nrLSU in section Caesareae.

With regard to the nrITS sequences, in comparison with a recently obtained complete sequence, the genetic distance is very large — about 37%. The Sanchez nrITS sequences (they really weren’t part of his project and didn’t receive a lot of attention for that reason) are incomplete and show a 21+% genetic distance.

So, this seems to be something quite distinct from calyptratoides.

On this page:

all the sequences we have for calyptratoides based on material from the Roosevelt herbarium are accessible from GenBank by clicking on the GenBank accession numbers in the “GenBank nos.” data field near the top of the technical tab.

Very best,


I went back this year,
By: Noah Siegel (Noah)
2015-01-03 08:51:13 PST (-0800)

looking for more. Although I didn’t find anything under these trees, I did find Amanita calyptratoides under some nearby oaks.

How does that compare to this micro/DNA wise. Macro is off, but these were beat-up specimens.

I should also say…
By: R. E. Tulloss (ret)
2015-01-02 21:33:12 PST (-0800)

that since Sanchez et al. just published research that resulted in many nrLSU sequences from section Caesareae being posted to GenBank, it is worth more than chopped liver to say that the nrLSU sequence derived from this material found no match in GenBank.


We were able to get a partial forward read of “proposed fungal barcode” &
By: R. E. Tulloss (ret)
2015-01-02 21:29:45 PST (-0800)

a good segment of the nrLSU gene.

Congratulations on getting this critter to the dryer in time to make it possible to get some DNA without too much trouble.

I was very surprised when all of the closest matches in GenBank (none a perfect match) were to species of section Caesareae. Maybe there was once a ring on those stems? At any rate, the pallidity of the spores in Melzer’s reagent is supported by placement of the species in subgenus Amanita. Quite a few surprises from this material.

I think the DNA is certainly solid enough to be able to recognize this taxon if you find it again.

It would be very interesting to know more about this species.

Very best,


Thanks, Noah.
By: R. E. Tulloss (ret)
2014-04-26 11:16:31 PDT (-0700)

We have accessioned this material in the herbarium in Roosevelt.

Thank you also for the email that pointed me to this observation. You mentioned in the email that the material was a bit old when collected and was dried quickly in an attempt to salvage what could be salvaged.

There sure was something strange going on because the spores are very irregular in shape. They are long enough to match A. fallax, but the lengths are all over the map. In the first section of gill that I tried, there were many irregularly shaped immature spores and not many mature ones. This could be due to senescence, which is suggested by your comments on the mushrooms’ condition.

If I can make more of this material, I’ll be sure to let you know. It’s very curious.

Very best,


I haven’t seen a specimen for many years, but…
By: R. E. Tulloss (ret)
2014-01-15 18:22:07 PST (-0800)

Just as a shot in the dark, could this be Amanita fallax?

I’d love to see a mature specimen.