When: 2014-08-05

Collection location: Harvey’s Lake, Pennsylvania, USA [Click for map]

Who: Dave W (Dave W)

No specimen available


Proposed Names

58% (1)
Recognized by sight
29% (1)
Recognized by sight

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= Observer’s choice
= Current consensus


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Hi, Linas. Same here.
By: R. E. Tulloss (ret)
2014-08-07 07:43:13 PDT (-0700)

My experience so far is that there is no appreciable (and repeatable) difference in either…the nrITS (“proposed barcode”) region or the nrLSU gene.

Very best,


Indeed something interesting …
By: Linas Kudzma (baravykas)
2014-08-07 06:11:11 PDT (-0700)

I do not think I have mentioned this on MO but my observations http://mushroomobserver.org/140380 and http://mushroomobserver.org/140549 have identical ITS sequences. One is dark gray and the other almost pure white. Both are a 100% match to GenBank KC855217 Amanita brunnescens voucher RET 031-1

I’ve been talking with my son, David. EDITED.
By: R. E. Tulloss (ret)
2014-08-07 03:47:39 PDT (-0700)

About the idea of adding another set of tabs on our taxon page so that different distinctive color variants could be called out within what current genetic information indicates may be a single species. This would apply in the case of the names brunnescens and aestivalis. The first is the older name and has priority if the two names are taxonomic synonyms.

At the very least there is some difference in chemistry…apparently associated with pigment development in the cap (barely happening) and staining/bruising happening slowly as far as the human eye is concerned.

There’s something interesting going on there. A pigment precursor missing or in limited supply? I know from the past, that even in strongly staining brunnescens, adding phenolic compounds that should trigger a pigment production cycle via the enzyme tyrosinase produces no result. If you add the some phenolic compound to rubescens or almost any species of sect. Vaginatae or sect. Caesareae (and many other amanitas) you get a strong orange-red to red-brown reaction within less than 15 minutes. So there is something curious about pigment in brunnescens anyway…even before the issue of low or non- pigment production in aestivalis shows up.

Here’s an interesting project…

Very best,