When: 2014-08-20

Collection location: Gainesville, Florida, USA [Click for map]

Who: Richard Kneal (bloodworm)

Specimen available

Images

Proposed Names

29% (1)
Recognized by sight
ret
55% (1)
Recognized by sight

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= Observer’s choice
= Current consensus

Comments

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Thank you for the additional images, Richard.
By: R. E. Tulloss (ret)
2014-08-21 03:38:15 PDT (-0700)

I think the very narrowly fusiform elements are something foreign to Amanita tissue. It’s something else from the environment.

The tissues of the volva on cap and stem (if this is an Amanita) will be rehydratable after drying. It does look as though there are amyloid spores (broadly ellipsoid to ellipsoid) in several of the images. That would eliminate a species with dominantly globose to subglobose spores in Amanita, such as A. thiersii (which also has a skirt on the upper stem).

I would suggest using less material in your mounts. Make very thin slices of gills…as thin as you can make them and spread them out a bit more. Mere whisps of the volva (from below the very surface to avoid the cells most destroyed by decay or drying) will do in order to see the cell structure I mentioned.

Very best,

Rod

ret…
By: Richard Kneal (bloodworm)
2014-08-20 22:27:55 PDT (-0700)

surely.

i tried to look at the volval material, but i unfortunately saw your message after i had it in the dryer and not much material remained.

i posted some micrographs.
i can get measurements tomorrow.

i guess the spores could be described as “pseudoamyloid.”
majority of basidia was 2-spored…however, i may have seen a couple that were 4-spored.

[edit]: im not really sure what the 3rd and 4th micrographs represent.

thanks.

If it is convenient to share part of the material,…
By: R. E. Tulloss (ret)
2014-08-20 18:46:21 PDT (-0700)

I’d love to look at it.

Very best,

Rod

Proportionately fatter cells would indicate something closer to chlorinosma or longipes.
By: R. E. Tulloss (ret)
2014-08-20 18:45:20 PDT (-0700)

Nice photos.

Very best,

Rod

Hello, Richard.
By: R. E. Tulloss (ret)
2014-08-20 18:43:59 PDT (-0700)

I can’t remember if you ever post photomicrographs.

This might be a case where a mount of volva from the cap or stem spread out very thinly and mounted in water (a drop of alcohol or a little heat will help get rid of bubbles in the mount) would tell us a good deal. Lots of hotdog or sausage shaped cells in chains would indicate that you have something from the non-mycorrhizal group.

Very best,

Rod