Notes:
Growing on a mossy patch in tall grass on a parcel of land between a dirt road a row of young pitch pines; the were a couple of oak saplings nearby.
Too young to drop spores — the pore mouths were closed.
Cap = 3.8 cm (1.5") in diameter; overall fruitbody height = 5.7 cm.
The pore surface didn’t stain any color regardless of what the pix show.
The cap context stained blue above the tubes and red below the pileipellis.
This material has been preserved.

[Original ID assessment before DNA data and additional collections from the Gulf states became available] The possibility of this bolete being B. patrioticus, as suggested by Dr. Bessette, is intriguing. The fact that we do find quite a few “southeastern” basideomycetes in the NJ Pine Barrens (e.g., Boletus oliveisporus, Austroboletus subflavidus and Amanita williamsii) supports the hypothesis that B. patrioticus might be found to the north of its previously reported northernmost distribution range (NC west to OH). The mixed conifer-hardwood habitat of the Barrens is rather unusual for this find, but young oaks were observed in the vicinity. As far as the specimen itself is concerned, its gestalt appearance is similar to the boletes identified as B. patrioticus in obs 34091, obs 95107, as well to the corresponding photos in North America Boletes by Bessette et al. The tri-color context pattern in the cap is indicative, too. Lack of bluing on the pore surface of my specimen, however, is not in agreement with the description. Could bluing be a function of the age?

DNA Sequencing Discussion (last updated 29-Nov-20):
> A clean and contiguous nrDNA (SSU-ITS-LSU) sequence of 2215 bps was derived from this voucher by Dr. Kudzma.
The full length ITS sequence is 618 bps long in this organism. It is a >99% match to an uncultured ectomycorrhizal fungus clone (https://www.ncbi.nlm.nih.gov/nuccore/KC424548; an ITS-LSU sequence). It is also a good match (>99% similar) to the more recent ITS sequences of obs 320304 (from LA) and obs 338178 (from TX).
The ITS is followed by the first 1447 base pairs (bps) of the nrLSU region. A BLAST of the first 963 characters of the LSU sequence gave a 100% match with #KC424548 (the same accession from the ITS BLAST), as well as a 99.9% match with the accession of ‘B. communis voucher’ from North Carolina (https://www.ncbi.nlm.nih.gov/nuccore/AY612801). According to Dr. Alan Bessette, B. communis is an old and incorrect name for B. patrioticus (see http://fm1.fieldmuseum.org/nama/?page=view&id=811).
> Most recently (late Nov. 2020), a clean a contiguous TEF-1 sequence became available. It’s derived from a forward and reverse reads, and covers 924 bps immediately downstream of the EF1-983F primer, which was seen but edited out. This entire sequence is 99.5% similar to the slightly longer TEF-1 of MO338178 from TX. Despite the 5 mismatching positions, there is no question the two collections are conspecific.
The reason behind TEF-1 sequencing was to seek a better phylogenetic resolution typically offered by single-copy protein-coding genes after the nrDNA data turned out totally uninformative in this regard. This strategy may have paid off following my analysis of pertinent BLASTn data. A carefully annotated phylogenetic tree generated in GenBank using relevant accessions derived from a BLASTn-500 of the first 588 nucleotides from the sequence of the TX collection, obs 338178, that represent the most commonly sequenced region in TEF-1 (as far as GenBank data are concerned) has already been uploaded to this post. Therein, B. patrioticus is shown to clade in the subfamily Boletoideae, where it appears to be a member of its own (monotypic) genus. IMO, the unique morphological profile of this taxon is in line with the new genus theory.
Conclusions: 1) Based on the above evidence, this bolete in all likelihood represent the first reported record of B. patrioticus from NJ. DNA data from the B. patrioticus holotype (currently located at the Buffalo Museum of Science) would be desirable for an unambiguous confirmation of the species concept for this unique bolete.
2) Based on the phylogenetic analysis of the TEF-1 sequence, B. patrioticus is positioned as a unique lineage in Boletoideae.

Species Lists

Images

No flash; taken 20 min before sunset
Taken with a flash
Taken with a flash
No flash
No flash
No flash
Fig. 1: TEF-1 tree derived from the sequence of B. patrioticus in obs 338178

Proposed Names

29% (1)
Recognized by sight
83% (7)
Used references: Suggested by Alan Bessette (personal communication)
Based on chemical features: 1) The derived ITS sequences is >99% similar to those of obs 320304 from LA (#MN128240) and obs 338178 from TX
2) The derived TEF-1 sequence is 99.5% similar to that of obs obs 338178 from TX

Please login to propose your own names and vote on existing names.

= Observer’s choice
= Current consensus

Comments

Add Comment
Molecular Data Discussion Posted
By: I. G. Safonov (IGSafonov)
2015-05-16 08:09:31 CST (+0800)
Hello, Dave
By: I. G. Safonov (IGSafonov)
2015-01-05 08:39:41 CST (+0800)

Happy New Year! Thank you for posting your comment in support of B. patrioticus.

AuroraS,
By: I. G. Safonov (IGSafonov)
2015-01-05 08:36:20 CST (+0800)

Thank you for pointing out B. harrisonii as a possibility. I’ve never heard of this name before, and your proposal prompted me to investigate the subject and get a complete description of this mushroom on MushroomExpert.com and other hot-of-the-press updates on boletes on that website, like the key to 30+ species of red-capped bluing boletes and a careful tabulated comparison between bicolor, sensibilis and psedosensibilis among others. But I am digressing…

I don’t think it fits the bill here for a variety of reasons, such as a more stocky appearance and lack of bluing on the pores and other external surfaces, for starters. The diminutive size is due to immaturity and dry conditions, and not necessarily attributed to genetics. The campestris-fraternus-rubellus-subfraternus cluster, to which B. harrisonii appears to belong, is a readily recognized group consisting of gregarious, oak-associated, small-sized and graceful red-capped and readily bluing boletes, some with a “xerocomoid” hymenophore. I am quite familiar with the group, but I have never collected any one of those in the Barrens, which appears to be the wrong habitat for this group probably because of the soil. To my bolete-trained eye, this specimen looked rather unique from the first moment I saw it. The only species that it reminds me a bit of is Boletus miniato-olivaceus I collected earlier that year under hemlock (obs 168189).

Despite the questionable distribution range and lack of surface bluing, B. patrioticus is still a viable possibility. If there exists a vouchered specimen that has been sequenced (nrITS or nrLSU), I would be willing to submit my sample for analysis. At the same time, I don’t exclude the possibility of this being an undescribed taxon given my extensive experience of collecting and documenting boletes of the NJ Pine Barrens. I will be on a look out for a larger and age-diverse collection of this mushroom when the time comes…

Reading the BRB account…
By: Dave W (Dave W)
2015-01-05 07:48:04 CST (+0800)

of B. patrioticus, the description of the context within the cap stands out, “pale yellowish, pinkish red to purplish under the pileipellis or extending throughout the pileus, slowly staining blue below the reddish area when exposed.” This appears to be an exact match for the mushroom seen here. Moreover, the name “patrioticus” is derived from this red, blue, and whitish/yellowish context color contrast.

I have never forayed in the Pine Barrens. But from what I’ve seen posted here on MO, it would appear to be ill advised to rule out IDing a PB collection because the range does not extend as far north as this quite unique area.

I have found this one before, good luck trying to nail down an ID.
By: AuroraS
2015-01-05 04:51:04 CST (+0800)

It’s probably more likely that it’s not B. patrioticus if it’s both missing some of the B. patrioticus features and outside of its normal range. I have found this one before—cute and small, velvety cap, on the edge of a mixed hardwood forest in the grass, Mid-Atlantic US, resembling B. campestris et al., sort of but not really bruising blue, and won’t put out a spore print to save its life. Nobody could tell me what the damn thing was, good luck.