Collection location: Norwich, Connecticut, USA [Click for map]
out for sequencing.
|I’d Call It That||3.0||10.24||2||(the3foragers,IGSafonov)|
sum(score * weight) /
(total weight + 1)
I find your explanation satisfactory. :-)
I am not so much interested in figuring out what’s chippewaensis, clavipes and edulis in the field. Even if they are all different by DNA (other than the ITS), morphological plasticity is likely to hamper reliable identification. There will always be doubt. Ditto for the variipes group, though I am still very curious about what B. variipes Peck turns out to be by DNA. I think we will find out soon enough!
But you called obs 278521 chippewaensis, citing “variable color morphology” and that one is conspecific with 205929. :-) Please explain.
I do agree with you, Herbert, in that edulis s. str. should be screened against a panel of loci for a better species resolution. Other taxa in porcini s. str., with exception of the “pinophilus group” ITS resolves reasonably well, but additional genes may enhance the picture of evolutionary relationships in the main porcini lineage.
I thought you called all NE edulis types with variable cap colors chippewaensis? This one has variably-colored caps, does it not? :-)
You would have to talk to Dr. Dentinger about sampling a multitude of porcini taxa for 2-5 loci. Get you checkbook ready! :-)
Three sequences have been obtained from this material and posted to the observation:
1) The nrITS read is 664 bp long. It starts in the ITS1 primer and ends just 7 bps shy of the beginning of nrLSU region. There are two ambiguous characters: a “Y” (C/T) in the ITS1 region and an “R” (A/G) in the ITS2 region. I edited out the last 7 bases because of the editing errors present therein.
2) The nrLSU read is 951 bps long. It starts in the ITS4 primer and ends in the LR5 primer. There is a single ambiguity, an “R”.
3) A TEF-1 sequence fragment is 946 bps long. The region between and including the EF1-983F and EF1-1567R primers is 613 nucleotides long. 261 bps precede the EF1-983F primer and 72 bps follow the EF1-1567R primer. The sequence ends in the first two characters of the tef1R primer.
Comparison of the ITS and TEF-1 sequences with those of obs 278521 (also by Robert) shows them to be perfectly identical sans the SNP ambiguities present in both. As far as I am concerned, these two collections are conspecific. Sequencing results of 278521 have already been discussed by me.
Herb Baker (Erlon) would call this B. chippewaensis. :-) However, according to the research by Dr. Bryn Dentinger at the Natural History Museum of Utah, that species should be synonymized with edulis, while B. edulis var. clavipes should be elevated to the species rank, i.e., B. clavipes.