Notes:
primarily Oak forest. Kind of reminds me of Amanita magna, the large specimen very large a foot tall. They all were found independently of one another, but I grouped them for progressions sake. I am not 100% sure of the I.D. but none of the other Vagniatae around today were of the sturdy nature of these mushrooms.

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Not contaminated. GenBank response is that differs in 4-6 character (of 1454)..
By: R. E. Tulloss (ret)
2019-09-20 19:33:37 PDT (-0700)

from sp-AZ31 (which is only known from the SW U.S.). Packing in for the night.

R

The link to NIH is down at the moment so I have to BLAST against my local data base.
By: R. E. Tulloss (ret)
2019-09-20 19:19:01 PDT (-0700)

That would be OK unless the sequence from the present material is contaminated. My data bases contain only amanitas. So of course the closest sequence will be a sequence from an Amanita.

I’ll try GenBank again in a little while.

Very best,

Rod

Consequently if I had a sample of Brewer’s Yeast it would come out as distant from some Amanita. I’ll be back.

Rod

The sequence available from a Lamoureux collection of A. magna differs…
By: R. E. Tulloss (ret)
2019-09-20 18:55:36 PDT (-0700)

from or nrITS sequence derived from the present material by nearly 20%. Amanita magna does have the penetratrix type nrLSU initial motif. I have not come to grips withthe very large genetic distance reported above. There must be more ambiguity that I don’t immediately see in the alignment. I just see mismatches of some type. Too late to dig into this deeply tonight.

Very best,

Rod

The species may be a new one.
By: R. E. Tulloss (ret)
2019-09-20 18:35:04 PDT (-0700)

The nrLSU sequence has a small region which does not sequence cleanly because forward and reverse reads do not provide consistent or good quality data in that region. This, in itself, is a character of the DNA and could be consistent across all specimens, but that is something we can’t tell yet (of course).

The initial motif of nrLSU is of the penetratrix type.

Can you help me with interpretation of the cap colors in the photographs, please?

Very best,

Rod

A pretty good nrLSU sequence has been derived from the voucher of this observation.
By: R. E. Tulloss (ret)
2019-09-20 18:28:22 PDT (-0700)
Thanks Garrett,
By: groundhog
2015-07-21 08:23:29 PDT (-0700)

We have received this material and it has been accessioned to Rod’s herbarium. We have scheduled it for DNA sequencing.
-Naomi

I’ll bite.
By: R. E. Tulloss (ret)
2015-07-13 03:41:09 PDT (-0700)

I don’t recognize them either, Garrett. If you can spare part or all of the collection, I would like to include these in my research.

Very best,

Rod