General Info:
> Collected (not by me) at the COMA Foray (forage site #8)
> Small and dark fruiting bodies; caps ~1.25" in diameter
> Unknown habitat, but presumably of mixed coniferous woods
> Cap flesh slowly staining blue
> The ID sticker says nebulosus, but this is likely to be incorrect

DNA Discussion (updated/edited on 11-Apr-17):
> A clean and contiguous nrLSU sequence of 1461 bps was obtained from this material. There are three ambiguous characters: one “R” and two “Y”.
> A BLAST search of the full-length sequence gave a 99.6 % match with Porphyrellus porphyrosporus #DQ534643 (1413/1419; 97% query cover). The associated reference = “Molecular systematics and biological diversification of Boletales” Mycologia 2006, 98 (6), 971-981. This was the only high-scoring hit at this query length that made sense.
> A BLAST search of the first 975 characters returned the following profile (sorted by % identity):
Porphyrellus pseudoscaber #EU685107 (USA) = 949/954 = 99.5%
Tylopilus porphyrosporus #HQ161850 (USA) = 838/843 = 99.4%
Porphyrellus porphyrosporus #DQ534643 (Germany; same as above) = 927/933 = 99.4%
Tylopilus porphyrosporus #KF112482 (China) = 842/848 = 99.3%
Tylopilus porphyrosporus #KT990544 (China) = 841/848 = 99.2%

> Though none of the GB sequences were a perfect match, it’s possible that the nrLSU of porphyrosporus is more variable than one would anticipate for this conserved locus. Alternatively, it might a be a complex of closely related taxa that spans the globe.

Species Lists


Proposed Names

29% (1)
Recognized by sight
57% (1)
Based on chemical features: Matching LSU sequence in GenBank — DNA discussion is posted in the notes section

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= Observer’s choice
= Current consensus


Add Comment
Thank you, Dave and John
By: I. G. Safonov (IGSafonov)
2016-01-05 12:48:16 CST (+0800)

It’s hard to believe that no LSU sequences from P. porphyrosporus from the USA were recovered from the GenBank nucleotide search. Perhaps none exist as all NA collections have been sequenced only for ITS instead. The matching sequence from the German collection was used by both Nuhn et al. and Wu et al. in their recent phylogenetic papers on the Boletaceae from 2013 and 2014, respectively. This fact adds credence to this result and ID. In addition, Nuhn lists another LSU sequence (KF030346) that came from a second German collection of P. porphyrosporus, but for some unknown reason this one didn’t get picked up by the BLAST search. Roy Halling suggested I submit the LSU sequence from my material to GenBank as there is no coverage for NA specimens. I heard the submission process hadn’t been a trivial task, but maybe things got simpler over time. This is something to think about…

very nice
By: John Plischke (John Plischke)
2016-01-05 11:47:14 CST (+0800)

Very nice collection and notes

Nice job, Igor.
By: Dave W (Dave W)
2016-01-05 08:50:54 CST (+0800)

I often wonder about the possibility that eastern NA edulis material represents an array of native NA and imported Euro species. So, maybe this hypothesis also applies to other groups (clades?) of boletes?

DNA discussion posted
By: I. G. Safonov (IGSafonov)
2016-01-04 14:20:28 CST (+0800)